Characterisation of Multidrug-resistant Alcaligenes faecalis Strain AF1 Isolated from Patient of RUTIs: A Study from Bangladesh KC01-KC04
Dr. Md Javed Foysal,
PhD Research Fellow, School of Molecular and Life Sciences, Curtin University, WA-6845, Perth, WA, Australia.
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Introduction: Alcaligenes faecalis is a common environmental bacteria that often infects human as an opportunistic pathogen. It rarely causes Urinary Tract Infection (UTI) in human; however, infection brings severe outcomes. Also, the treatment of A. faecalis associated infections with common antibiotics can be difficult due to a high level of antibiotic resistance.
Aim: Molecular characterisation of A. faecalis isolate from women suffering from Recurrent Urinary Tract Infections (RUTIs).
Materials and Methods: The study has been conducted in the USDA-laboratory of the Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. The present study conducted from July 2017 to December 2017, characterised an A. faecalis strain from the women suffering from RUTIs by applying 16s rRNA gene sequencing and phylogenetic analysis. Antibiogram profiling was done by means of disk diffusion assay. Analysis of in-silico SNP variants was done using Geneious software.
Results:The isolates were resistant to seven out of ten commercial antibiotics used to treat UTI in Bangladesh. Phylogenetic and evolutionary distance data analysis revealed a close proximity of the study AF1 strain with other A. faecalis strains identified from the environment, especially from a previously characterised water sample. In-silico variants search found nine potentials of Single Nucleotide Polymorphism (SNPs) in the studied strain compared to other environmental A. faecalis bacteria characterised from India, South-Korea, Japan, Mexico and Brazil.
Conclusion:The present study revealed the transmission of environmental opportunistic pathogens to human and cause chronic infections in Bangladesh.