Correlation of HDL Associated Paraoxonase 1 with Oxidative Stress Markers in Hypertensive Dyslipidemic Patients BC23-BC26
Dr. Sudeep Kumar,
Research Scholar, Department of Biochemistry, Santosh Medical College, Muzaffarnagar-201009, Uttar Pradesh, India.
Introduction: Paraoxonase 1 (PON 1) is an esterase, exclusively synthesised by the liver and a high density lipoprotein-associated antioxidant enzyme.
Aim: To determine the PON 1 and oxidative stress markers and to find out the possible correlation between them in hypertensive dyslipidemic patients.
Materials and Methods: This study included 500 patients, out of which 250 were diagnosed with hypertension and the remaining 250 acted as controls age and sex-matched individuals. PON 1 activity was measured manually by using spectrophotometer, Malondialdehyde (MDA) by TBA method, Superoxide Dismutase (SOD) by Marklund and Marklund, lipid profile was measured by biochemistry auto-analyser. The statistical differences between cases and controls were determined by independent studentâ€™s t-test.
Results: There were significantly increased levels of PON 1, vitamin E and C and SOD in hypertensive subjects as compared to controls. With respect to lipid profile, significantly increased levels of total cholesterol, triglyceride, Low Density Lipoproteins (LDL-c), Very Low Density Lipoprotein (VLDL-c) and decreased level of High Density Lipoproteins (HDL-c) in cases as compared to controls were observed. Moreover, PON 1 statistically and positively correlated with HDL-c, SOD, vitamin E and C and it negatively correlated with MDA.
Conclusion: It concludes that, in hypertension which is caused due to the interplay of several confounding factors such as oxidative stress, increased oxidised LDL lipoprotein and decrease in the level of antioxidants, the PON 1 activity is found to alter.