Prevalence of Class I and II Integrons among MDR Enterobacter cloacae Isolates Obtained from Clinical Samples of Children in Kermanshah, Iran DC13-DC16
Dr. Kamal Ahmadi,
Research Assistant, Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences,
Shirudi blvd, Parastar blvd, Postal code: 6714869914, Kermanshah, Iran.
E-mail: email@example.com; firstname.lastname@example.org
Introduction: Enterobacter species are among one of the key causes of hospital infections. The transfer of drug resistance genes through the integrons promotes the development of antibiotic resistance and the emergence of Multidrug Resistant (MDR) strains.
Aim: The aim of this study was to determine the prevalence of class I and II Integrons among MDR Enterobacter cloacae isolates obtained from clinical samples of children in Kermanshah, Iran.
Materials and Methods: This descriptive cross-sectional study was done during 11 month period from October 2016 to September 2017, 72 isolates of E.cloacae were collected from children under 15 years of age in Mohammad Kermanshahi Hospital in Kermanshah, Iran. After confirmation of the isolates with biochemical specific tests, their antibiotic susceptibility with disk diffusion was examined. Then the frequency of Class I and II integrons was determined by using their specific primers and by PCR method. Data was analysed by SPSS software version 20 and p-values less than 0.05 were considered statistically significant.
Results: The highest and lowest frequency of isolates were in blood samples 29 (40.3%) and CSF 2 (2.8%) respectively. From the 72 isolates of E.cloacae, 54 isolates (75%) were MDR. The highest antibiotic resistance was observed against Ampicillin-clavulanic acid (94.4%) and Cefalexin (69.4%), whereas the lowest antibiotic resistance was to Imipenem (9.7%) and Colistin (6.9%). Genotypically, the frequency of class I integron was 42 (58.3%), but none of the isolates had class II integron.
Conclusion: The results of this study demonstrates that E.cloacae isolated from children, in addition to the high frequency of MDR isolates, the prevalence of isolates with integron is expanding. Therefore, keeping with the role of integrons in resistance to different antibiotics, it is necessary to pay greater attention to identify them.