Alternative Rapid Methods for Coverslip Removal: A Comparative Study EC01-EC02
Mr. Sachin Shivaji Kapse,
Postgraduate, Department of Pathology, B.L.D.E University, Shri. B.M. Patil Medical College, Vijayapura-586103, Karnataka, India.
Introduction: In 21st century, the focus is to develop method which yields outcome in shorter tenure, and are rapid, safe and obtain quicker results. In this study, non-traditional methods were used for removal of coverslip and was compared with traditional methods.
Aim: To compare the time taken for coverslip removal of old faded slides with five different methods.
Materials and Methods:Faded slides of one year to more than 10-year-old were included in the study. Total of 90 slides were subjected to five different methods of coverslip removal like xylene at room temperature, xylene at 56° Celsius (C), Petrol, Diesel and Freezing method. Time taken for each method was noted.
Results: The mean time taken for removal of coverslip was least with freezing method followed by xylene at 560C, Petrol, xylene at room temperature and diesel.
Conclusion: Alternative methods are safe, rapid and has less turn around time compared to routine use of xylene. Hence, can be successfully used for removal of coverslip.