Comparative Evaluation of Serum Malondialdehyde (MDA) Level in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma ZC27-ZC31
MIG-181/12, Avas Vikas, Rudrapur, Uttarakhand, India.
Introduction: Lipid peroxidation which is induced by reactive oxygen species is involved in the pathogenesis of malignancy. This lipid peroxidation levels are indicated by Malondialdehyde (MDA), which is the most frequently, used biomarker for the detection of oxidative changes.
Aim: Comparative evaluation of serum MDA level in Oral Submucous Fibrosis (OSMF) and Oral Squamous Cell Carcinoma (OSCC) patients and comparison of their serum MDA levels with healthy controls.
Materials and Methods: The study included 150 patients comprising 50 apparently healthy controls without any tobacco chewing habits, 50 clinically diagnosed patients with OSMF which were also subgrouped according to Interincisal Opening (IIO) and 50 clinically diagnosed patients with OSCC, they were also subgrouped according to site, size and histopathological differentiation. Blood samples were obtained; serum was separated and evaluated for MDA levels which are the measure of oxidative stress by using principle of spectrophotometry at 532 nm absorbance. Statistical analysis were conducted using independent t-test and one-wayanova test. Statistical package for social science (SPSS 16) was used for the analysis.
Results: The mean serum malondialdehyde level in the control group was found to be 10.50 nmol/mL, whereas it was 25.87 nmol/mL and 57.00 nmol/mL in OSMF and OSCC, respectively. The study revealed enhanced MDA levels in serum of OSMF and OSCC patients as compared to controls. Different grades of OSMF patients according to IIO showed p<0.05, which was statistically significant. Among the subgroups of OSCC patients with respect to site and histopathological differentiation the results were not significant and there was statistically significant increase in MDA levels with increase in primary tumour size.
Conclusion: The increased level of MDA reflects the extent of lipid peroxidation and is considered to be mutagenic as well as carcinogenic and can also modulate the expression of genes related to tumour promotion.