Bacteriological Analysis of Water Samples from Different Points in a Tertiary Care Hospital DC04-DC09
Mr. Yashraj Kirankumar,
112, Surabhi, 3rd Main, 5th Stage, BEML Layout, Next to Swargarani Church, Rajarajeshwari Nagar, Bangalore-560098, Karnataka, India.
Introduction: Water is a critical component of public health, and failure to supply safe water will place a heavy burden on humanity. Drinking water is the major source of microbial pathogens in developing regions. Water contamination can be a major source of infection in a hospital.
Aim: To determine the percentage of contamination (if contamination is present) with calculation of prevalence of bacteria and to analyse the bacteriological profile of the organisms present.
Materials and Methods: Hi Media test kit was used to collect water samples from 20 different points in the hospital and was subjected to incubation for 24-48 hours. The bottles that showed colour change were further subjected to culture and sensitivity with identification of organism.
Results: There was 100% contamination of the water samples and the organisms identified include E.coli, Klebsiella, Enterobacter, Citrobacter, Proteus, NF GNB, Salmonella, Pseudomonas and Acinetobacter. Some of these organisms and isolates were found to be resistant to Cefuroxime, Ampicillin, Amoxicillin- clavulanic acid, Cefoperazone, Imipenem.
Conclusion: These results provoked the authors to reconsider the efficacy of the existing water purification systems and analyse where the point of contamination is, take corrective measures and use the apt method of purification systems to improve the quality of life of the patients.