Comparative Evaluation of The Efficacy of Aloe Vera Gel with Milk and Hank’s Balanced Salt Solution in Maintaining the Viability of PDL Cells in Avulsed Teeth ZC11-ZC15
Dr. Parvathy Kumaran,
Professor, Department of Pedodontics and Preventive Dentistry, Amrita School of Dentistry, Amrita Vishwa Vidyapeetham, Amrita Institute of Medical Sciences, Ponekkara, Kochi-682041, Kerala, India.
Introduction: Various storage media have been investigated due to their ability to maintain the viability of the Periodontal Ligament (PDL) cells, thus permitting longer extra-alveolar periods prior to replantation of avulsed teeth.
Aim: To assess the efficacy of a newer storage medium, Aloe Vera Gel in comparison with milk and Hankâ€™s Balanced Salt Solution (HBSS) in maintaining the viability of PDL cells in simulated avulsed teeth.
Materials and Methods: The present in-vitro study was performed on 40 freshly extracted human premolars with intact crown and closed apices, extracted for orthodontic reasons. They were randomly assigned to one of the three storage medium groups (N=10 each) and a positive and negative control (N=5 each). The positive and negative controls corresponded to 0 minute and an eight hours dry time respectively. The teeth in the experimental group were dried at room temperature for 30 minutes followed by 45 minutes immersion in one of the three experimental media. After drying and soaking, the root surface of each tooth was treated with an enzymatic solution containing 1 mL of Collagenase Type II and Dispase Type I in phosphate buffer saline (PBS). The cells were then labelled with 0.4% Trypan blue and counted using a haemocytometer under a light microscope at 10x magnification. Kruskal Wallis H-test was used to analyse the mean difference in the number of viable cells among the five groups.
Results: The teeth stored in HBSS demonstrated the highest number of viable PDL cells followed in rank order by milk, positive control, aloe vera and negative control. There was no significant difference in the number of viable PDL cells between HBSS and milk.
Conclusion: From the results of the present study it can be concluded that aloe vera may be better than negative control to maintain PDL cell viability but not more than HBSS and milk.