The Association between lasB and nanI Genes with Biofilm Formation in Pseudomonas aeruginosa Clinical Isolates DC01-DC03
Dr. Iraj Pakzad,
PhD., Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Introduction: Several acute and chronic infections in humans can be caused by Pseudomonas aeruginosa (P.aeruginosa). Biofilm formation by these bacteria threatens health setting via increasing resistance against antimicrobial agents.
Aim: To investigate the association between lasB and nanI genes with biofilm formation in P. aeruginosa clinical isolates.
Materials and Methods: A total of 161 P. aeruginosa clinical isolates were collected. The Microtiter Plate (MTP) method was applied for biofilm formation assay. Gene amplification was conducted by PCR method with specific primers.
Results: Present study findings showed that the prevalence of lasB and the nanI were 80.7% (130/161) and 24.8% (40/161), respectively. The biofilm formation results demonstrated that 15.5% (25/161) of isolates were not able to produce biofilm formation. It was considerable that the prevalence of lasB in P. aeruginosa producing biofilm was higher.
Conclusion: lasB can be considered as an effective factor for biofilm formation in P. aeruginosa isolates.