Utility of Histochemical and Immunohistochemical Profile in Grading of Squamous Cell Carcinoma of the Oral Cavity EC01-EC05
Dr. Samarth Shukla,
Professor, Department of Pathology, Jawaharlal Nehru Medical College, Wardha-442001, Maharashtra, India.
Introduction: Oral Squamous Cell Carcinoma (OSCC) accounts for approximately 90% of all the oral malignancies and a major cause of morbidity and mortality worldwide. The study utilises the Annerothâ€™s multifactorial grading system with Argyrophilic Nucleolar Organiser Region (AgNOR) score and the p53 expression as adjuncts; emphasising on the enhanced efficiency rendered by nuclear proliferative indices is the gold standard method of histomorphological grading of OSCC.
Aim: To analyse the relationship between the histomorphological grading (Annerothâ€™s grading) and the nuclear proliferative markers that is AgNOR and p53 protein in OSCC.
Materials and Methods: Total 98 cases of OSCC were investigated. The histomorphological grading was done according to the Annerothâ€™s system. The AgNOR score was quantified by counting the mean number of AgNORs per cell. The p53 expression was graded as score 0 to 3 according to the percent of cells showing positive p53 immunostaining.
Results: An increasing trend in the mean AgNOR count and p53 expression was observed with progressing histological grade of OSCC.
Conclusion: The nuclear proliferative indices (AgNORs and p53) serve as reliable predictive markers and can be used as an adjunct to the gold standard Annerothâ€™s histomorphological grading system of OSCC.