Comparative Evaluation of Antimicrobial Efficacy of Commercially Available Herbal Dentifrices against Selected Putative Oral Microorganisms: An In-vitro Study ZC18-ZC21
Sameer Anil Zope,
Department of Periodontology, School of Dental Sciences, KIMS Deemed to be University, Malkapur, Karad, Maharashtra, India.
Introduction: Dental plaque accumulation has been identified as the primary cause of dental caries, periodontal diseases, and halitosis. Worldwide, a wide range of commercially available nonherbal and herbal dentifrices are used along with tooth brush as mechanical aids in plaque control. Owing to some undesirable side effects associated with non-herbal toothpastes, there has been an increased popularity of herbal toothpaste among the general population.
Aim: To assess and compare antimicrobial efficacy of three commercially available herbal toothpastes against selected putative cariogenic, periodontal and fungal microorganisms.
Materials and Methods: An in vitro assessment of antimicrobial efficacy of Sudanta, Dant Kanti, Colgate Cibaca Vedshakti toothpastes was done during the months of February to March 2017 against pure culture standard strains of S. mutans, C albicans, P. gingivalis, A. actinomycetemcomitans and T.forsythia. All the selected microorganisms were sub-cultured on specific culture media. Antimicrobial efficacy of the toothpastes was then tested in triplicate at full strength using standard discdiffusion method. The antimicrobial efficacy was evaluated by measuring the zones of inhibition in millimetres surrounding disc containing the toothpastes. Means and standard deviations of the inhibitory zones were calculated for all herbal toothpastes and analysed using one-way ANOVA followed by Tukeyâ€™s posthoc tests for differences amongst the different groups. The p-value =0.05 was considered statistically significant.
Results: Sudanta and Dant Kanti toothpastes demonstrated higher antimicrobial activity against majority of the test microorganisms as compared to Colgate Cibaca Vedshakti toothpaste. Intergroup comparisons among the three toothpastes demonstrated that Dant Kanti toothpaste was significantly better in inhibiting S. mutans and C. albicans than Sudanta and Colgate Cibaca Vedshakti (p=0.05) while Sudanta toothpaste was significantly more effective against A. actinomycetemcomitans and T. forsythia than Colgate Cibaca Vedshakti toothpaste and Dant kanti (p=0.05). A. actinomycetemcomitans and T. forsythia were resistant to Colgate Cibaca Vedshakti toothpaste. However, Colgate Cibaca Vedshakti exhibited substantial antimicrobial activity against P. gingivalis when compared against Sudanta (p=0.05) and Dant kanti (p>0.05).
Conclusion: Results of the present study revealed that Sudanta demonstrated better antimicrobial activity against selected putative periodontal microorganisms: A. actinomycetemcomitans and T. forsythia. Dant kanti toothpaste demonstrated better antimicrobial activity against selected putative cariogenic and fungal microorganisms: S. mutans and C.albicans. Whereas Colgate Cibaca Vedshakti toothpaste exhibited highest antimicrobial activity against P.gingivalis