Biosynthesis and Characterisation of Ellagic Acid Coupled Silver Nanoparticles- An In-vitro Study ZC01-ZC05
Associate Professor, Department of Conservative Dentistry and Endodontics,
Vivekanandha Dental College for Women, Elayampalayam, Tiruchengode,
Namakkal, Tamil Nadu, India.
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Introduction: Nanomedicine dominated the world of therapeutics and diagnostics. Horizons are extending daily to identify a piece of the puzzle that helps us produce eco-friendly and cost-effective nanoparticles. In this, authors tried to synthesise a novel Silver Nanoparticle Coated with Ellagic Acid (EA-AgNPs) obtained from pomegranate using a simple method and explore its characterisation precisely.
Aim: To biosynthesise and assess the characterisation of Ellagic Acid coupled Silver Nanoparticles.
Materials and Methods: The study assessed the portrayal of EA-AgNPs were evaluated using various analytical techniques. Firstly, to analyse: the size of the EA-AgNPs using Ultraviolet (UV)-Visible Spectrometer; Secondly to determine the hydrodynamic size and its dispersity using Dynamic Light Scattering (DLS) and Zeta potential; thirdly to quantify the average size of EA-AgNPs by using Scanning Electron microscope (SEM); and lastly to identify the functional groups by using Fourier Transform Infrared Spectroscopy (FTIR) method. Descriptive statistics were used to analyse the results.
Results: Brownish colour change and shift of peak wavelength from 430 to 423 nm using UV-Visible Spectrometer analysis confirmed the formation and stability of EA-AgNPs. The DLS analysis revealed that EA-AgNPs were in nanosize (129.7 nm) with less aggregated polydispersity index (0.483). The Zeta potential confirmed that this newly synthesised nanoparticle was negatively charged -0.268mv. The SEM determination confirmed the formation of spherical-shaped nanoparticles with sizes ranging from 84.34-98.80 nm. The FTIR revealed EA-AgNPs exhibited different functional groups, which help to prevent particle aggregation.
Conclusion: In this work, the novel EA-AgNPs exhibited the apt characterisation needed for an effective and cost-efficient nanoparticle that could be effectively tapped in various fields of nanodentistry.