![](images/Newspaper.gif)
Evaluation of Anti-inflammatory, Antioxidant and Antimicrobial Activity of Pomegranate Peel Extract: An In-vitro Study
Correspondence Address :
Dr. Pradeep Dathan,
Research Scholar, Department of Prosthodontics, Saveetha Dental College, Chennai-600077, Tamil Nadu, India.
E-mail: pdathan70@gmail.com
Introduction: Pomegranate fruit contains biologically active compounds that provide anti-inflammatory properties. Byproducts of pomegranate and punicalagins inhibit the growth of pathogens while enhancing the growth of beneficial bacteria. The beneficial effects of phenolic compounds are exhibited in scavenging free radicals.
Aim: To evaluate the anti-inflammatory, antioxidant, and antimicrobial activity of Pomegranate Peel Extract (PPE).
Materials and Methods: The present in-vitro study was designed and carried out at the Nanobiomedicine Laboratort, Department of Pharmacology, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, India, from June 2021 to August 2021. The anti-inflammatory activity was evaluated using the Egg Albumin Denaturation assay (EA) and Bovine Serum Albumin Denaturation assay (BSA). The measurement of antioxidant activity was conducted using the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay, Hydrogen peroxide radical scavenging (H2O2) assay, and Ferric Reducing Antioxidant Power (FRAP) assay. Antimicrobial activity was evaluated using the agar well diffusion method. The microorganisms used to evaluate the antimicrobial effect of PPE were S. mutans, S.aureus, E.faecalis, and C.albicans. Results were analysed using independent t-tests, Analysis of Variance (ANOVA) and Tukey’s Honestly Significant Difference (HSD).
Results: Anti-inflammatory activity was observed with high concentrations of 40 and 50 μL in EA (70.06%±0.15, 78.08%±0.21) and BSA (75.50%±3.90, 80.82%±3.38) assays. Pronounced antioxidant activity of PPE was seen with higher concentrations of 40 and 50 μL in DPPH (88.17%±0.69, 92.50%±1.23), H2O2 (78.22%±0.94, 88.99%±1.03), and FRAP (78.43%±1.25, 88.49%±0.67) assays. The antimicrobial activity was highest at 100 μL for S.mutans (38±2.62 mm), S.aureus (36±3.16 mm), E.faecalis (21±1.48 mm), and C.albicans (23±2.36 mm).
Conclusion: The study concluded that PPE has anti-inflammatory, antioxidant and antimicrobial properties and these properties are concentration-dependent.
Assays, Flavonoids, Polyphenols, Punica Granatum L, Punicalgin
DOI: 10.7860/JCDR/2024/69878.19463
Date of Submission: Feb 03, 2024
Date of Peer Review: Feb 20, 2024
Date of Acceptance: Apr 16, 2024
Date of Publishing: Jun 01, 2024
AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? NA
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Feb 03, 2024
• Manual Googling: Apr 10, 2024
• iThenticate Software: Apr 13, 2024 (20%)
ETYMOLOGY: Author Origin
EMENDATIONS: 9
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2017: 134.54
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Embase
- EBSCOhost
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)
- www.omnimedicalsearch.com