Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 37448

AbstractMaterial and MethodsResultsDiscussionConclusionKey MessageAcknowledgementReferences
Article in PDF How to Cite Citation Manager Readers' Comments (0) Audio Visual Article Statistics Link to PUBMED Print this Article Send to a Friend
Advertisers Access Statistics Resources

Dr Mohan Z Mani

"Thank you very much for having published my article in record time.I would like to compliment you and your entire staff for your promptness, courtesy, and willingness to be customer friendly, which is quite unusual.I was given your reference by a colleague in pathology,and was able to directly phone your editorial office for clarifications.I would particularly like to thank the publication managers and the Assistant Editor who were following up my article. I would also like to thank you for adjusting the money I paid initially into payment for my modified article,and refunding the balance.
I wish all success to your journal and look forward to sending you any suitable similar article in future"

Dr Mohan Z Mani,
Professor & Head,
Department of Dermatolgy,
Believers Church Medical College,
Thiruvalla, Kerala
On Sep 2018

Prof. Somashekhar Nimbalkar

"Over the last few years, we have published our research regularly in Journal of Clinical and Diagnostic Research. Having published in more than 20 high impact journals over the last five years including several high impact ones and reviewing articles for even more journals across my fields of interest, we value our published work in JCDR for their high standards in publishing scientific articles. The ease of submission, the rapid reviews in under a month, the high quality of their reviewers and keen attention to the final process of proofs and publication, ensure that there are no mistakes in the final article. We have been asked clarifications on several occasions and have been happy to provide them and it exemplifies the commitment to quality of the team at JCDR."

Prof. Somashekhar Nimbalkar
Head, Department of Pediatrics, Pramukhswami Medical College, Karamsad
Chairman, Research Group, Charutar Arogya Mandal, Karamsad
National Joint Coordinator - Advanced IAP NNF NRP Program
Ex-Member, Governing Body, National Neonatology Forum, New Delhi
Ex-President - National Neonatology Forum Gujarat State Chapter
Department of Pediatrics, Pramukhswami Medical College, Karamsad, Anand, Gujarat.
On Sep 2018

Dr. Kalyani R

"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."

Dr Kalyani R
Professor and Head
Department of Pathology
Sri Devaraj Urs Medical College
Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka
On Sep 2018

Dr. Saumya Navit

"As a peer-reviewed journal, the Journal of Clinical and Diagnostic Research provides an opportunity to researchers, scientists and budding professionals to explore the developments in the field of medicine and dentistry and their varied specialities, thus extending our view on biological diversities of living species in relation to medicine.
‘Knowledge is treasure of a wise man.’ The free access of this journal provides an immense scope of learning for the both the old and the young in field of medicine and dentistry as well. The multidisciplinary nature of the journal makes it a better platform to absorb all that is being researched and developed. The publication process is systematic and professional. Online submission, publication and peer reviewing makes it a user-friendly journal.
As an experienced dentist and an academician, I proudly recommend this journal to the dental fraternity as a good quality open access platform for rapid communication of their cutting-edge research progress and discovery.
I wish JCDR a great success and I hope that journal will soar higher with the passing time."

Dr Saumya Navit
Professor and Head
Department of Pediatric Dentistry
Saraswati Dental College
On Sep 2018

Dr. Arunava Biswas

"My sincere attachment with JCDR as an author as well as reviewer is a learning experience . Their systematic approach in publication of article in various categories is really praiseworthy.
Their prompt and timely response to review's query and the manner in which they have set the reviewing process helps in extracting the best possible scientific writings for publication.
It's a honour and pride to be a part of the JCDR team. My very best wishes to JCDR and hope it will sparkle up above the sky as a high indexed journal in near future."

Dr. Arunava Biswas
MD, DM (Clinical Pharmacology)
Assistant Professor
Department of Pharmacology
Calcutta National Medical College & Hospital , Kolkata

Dr. C.S. Ramesh Babu
" Journal of Clinical and Diagnostic Research (JCDR) is a multi-specialty medical and dental journal publishing high quality research articles in almost all branches of medicine. The quality of printing of figures and tables is excellent and comparable to any International journal. An added advantage is nominal publication charges and monthly issue of the journal and more chances of an article being accepted for publication. Moreover being a multi-specialty journal an article concerning a particular specialty has a wider reach of readers of other related specialties also. As an author and reviewer for several years I find this Journal most suitable and highly recommend this Journal."
Best regards,
C.S. Ramesh Babu,
Associate Professor of Anatomy,
Muzaffarnagar Medical College,
On Aug 2018

Dr. Arundhathi. S
"Journal of Clinical and Diagnostic Research (JCDR) is a reputed peer reviewed journal and is constantly involved in publishing high quality research articles related to medicine. Its been a great pleasure to be associated with this esteemed journal as a reviewer and as an author for a couple of years. The editorial board consists of many dedicated and reputed experts as its members and they are doing an appreciable work in guiding budding researchers. JCDR is doing a commendable job in scientific research by promoting excellent quality research & review articles and case reports & series. The reviewers provide appropriate suggestions that improve the quality of articles. I strongly recommend my fraternity to encourage JCDR by contributing their valuable research work in this widely accepted, user friendly journal. I hope my collaboration with JCDR will continue for a long time".

Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
On Aug 2018

Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
The journal has a monthly publication and the articles are published quite fast. In time compared to other journals. The on-line first publication is also a great advantage and facility to review one's own articles before going to print. The response to any query and permission if required, is quite fast; this is quite commendable. I have a very good experience about seeking quick permission for quoting a photograph (Fig.) from a JCDR article for my chapter authored in an E book. I never thought it would be so easy. No hassles.
Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
My best wishes to Dr. Hemant Jain and all the editorial staff of JCDR for their untiring efforts to bring out this journal. I strongly recommend medical fraternity to publish their valuable research work in this esteemed journal, JCDR".

Dr. Mamta Gupta
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018

Dr. Rajendra Kumar Ghritlaharey

"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.
Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.

Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
Timely publication of journal: Publication of manuscripts and bringing out the issue in time is one of the positive aspects of JCDR and is possible with strong support team in terms of peer reviewers, proof reading, language check, computer operators, etc. This is one of the great reasons for authors to submit their work with JCDR. Another best part of JCDR is "Online first Publications" facilities available for the authors. This facility not only provides the prompt publications of the manuscripts but at the same time also early availability of the manuscripts for the readers.
Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
It is well said that "happy beginning is half done" and it fits perfectly with JCDR. It has grown considerably and I feel it has already grown up from its infancy to adolescence, achieving the status of standard online e-journal form Indian continent since its inception in Feb 2007. This had been made possible due to the efforts and the hard work put in it. The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. I must thank and congratulate Dr Hemant Jain, Editor-in-Chief JCDR and his team for their sincere efforts, dedication, and determination for making JCDR a fast growing journal.
Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."

Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
On May 11,2011

Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."

Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
On April 2011

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.

Dr. Anuradha
On Jan 2020

Important Notice

Original article / research
Year : 2007 | Month : June | Volume : 1 | Issue : 3 | Page : 131 - 137 Full Version

Serum Inhibin B: A Direct And Precise Marker Of Ovarian Function

Published: June 1, 2007 | DOI:

Department of Reproductive Biology All India Institute of Medical Sciences, New Delhi 110029. Email:

Correspondence Address :
Dr Ashutosh Halder, Associate Professor, Department of Reproductive Biology. All India Institute of Medical Sciences, New Delhi 110029
Tel: 011-26593304 , Fax: 011-26588663


Background Inhibin B is a glycoprotein hormone produced mainly by granulosa cells of the ovary in early folliculogenesis. It selectively suppresses the secretion of pituitary FSH and has local paracrine actions in the gonads. Its measurement is useful for investigating female reproductive dysfunction.
Objective The objective of this study was to examine serum levels of inhibin B in the assessment of ovarian function in patient with premature ovarian failure.
Material & Method Serum from premature ovarian failure (n=34; group A), menopause (n=8; group B) and normally cycling fertile women (n=5; group C) was prospectively collected and stored at –80°C. Serum concentration of inhibin B was measured using specific solid phase sandwich ELISA. FSH level was measured using microparticle enzyme immuno assay (MEIA) for comparison. Independent sample t test was used to see the mean significance differences between groups.
Results Inhibin B level was undetectable (i.e., <15pg/ml) in group A & B women. The mean value in group C women was 51.8pg/ml (range 26-75). Respective values of FSH were 78.8miu/ml (range 25-150), 100.7miu/ml (range 62-150) & 5.96miu/ml (range 4.2-7.9). Inhibin B level was significantly lower in group A & B than group C women (p<0.0001) whereas differences were insignificant between group A and B women. Similarly FSH level was significantly higher in group A & B than group C women (p<0.0001). We found wide variation in FSH level in group A women. In 5 women FSH level was below 40miu/ml and was related to exogenous estrogen intake more than 3 months of blood sampling.
Conclusion This study demonstrated that inhibin B is a better predictor for ovarian failure than FSH and uninfluenced by exogenous estrogen intake (if taken >3 months before).


Premature Ovarian Failure; Inhibin B & FSH

Inhibin B is a heterodimeric glycoprotein consisting one α-subunit and one βB subunit (1). Inhibin B expression and secretion in women is positively correlated with granulosa cell function, oocyte number & oogenesis (2),(3) and negatively correlated with FSH (4),(5),(6). It is regarded as a serum marker of oogenesis and may offer an improved diagnosis of ovarian function (7),(8).(9). Human FSH is a glycoprotein and consist of α and β subunits. In the workup of female infertility, FSH is the classical endocrine parameter to discriminate between ovarian impairment and other disorders. Several studies confirm that FSH level is a valuable
marker of oogenesis (10),(11),(12),(13),(14). The current study is designed to examine possible role of inhibin B as a predictor of ovarian reserve in comparison with FSH.

Material and Methods

The cases were selected from Department of Reproductive Biology, AIIMS between 2002 & 2003 while they were referred by various hospitals including our own for reproductive hormone analysis for infertility, menstrual irregularity or secondary amenorrhoea. Thirty four premature ovarian failure (POF) women (vide (Table/Fig 1) for diagnostic criteria) were selected for the study from 214 women referred. Suspected POF cases were interviewed in detail for medical, surgical & treatment (particularly exogenous estrogen & cytotoxic chemotherapy) history and examined clinically (general & systemic) to exclude any gross [Table/fig 1]underlying disease as per proforma. Most of the cases were investigated extensively outside (including ultrasonography, reproductive hormones, haemogram, biochemistry, TSH, ANA, etc) before attending our department. All related information was obtained in detail. Cases were selected as per criteria given in (Table/Fig 1).
No one had history of autoimmune disease, surgical removal of gonads, radiotherapy or chemotherapy. All the cases were followed up for at least one year. Five known normally cycling fertile women (group C) and eight menopausal women (group B) were included for comparison. Blood sampling from normally cycling fertile women was collected on day 2 or 3 of menstrual cycle. Postmenopausal women were selected from another ongoing project of the department. None of the cases had exogenous estrogen intake for 3 months before blood sampling although most were treated by estrogen in past (more than 3 months ago). Informed consent was obtained before blood sampling. All blood sampling were in fasting state. Serum was isolated after centrifugation and stored at -800C for 1 to 10 months before inhibin B & FSH assay. Hemolyzed and bilirubin containing samples were discarded from the study.

Serum concentration of FSH was measured using highly specific microparticle enzyme immuno assay (MEIA) using AxSYM automated immunoassay system (Abbott Laboratories, USA). Inter and intra-
assay coefficients of variation was <3%, cross reactivity with TSH, LH & hCG was <1% and detection limit was <0.4 mIU/ml. Serum concentration of Inhibin B was measured using a commercially available solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) specific for the dimeric inhibin-B (Oxford Bio-Innovation Ltd. Oxford, UK via Serotec) (15),(16). The first antibody is directed to the βB-subunit and the second antibody to the α-subunit and conjugated to alkaline phosphatase. The assay had a cross-reactivity of 0.1% with activin and ~1% with inhibin A. Assay sensitivity/detection limit was 15 pg/ml and the inter/intra-plate coefficients of variation was <7%. Before ELISA samples were pretreated with detergent (SDS), heated to 100ÂşC and exposed to hydrogen peroxide to enhance sensitivity as well as specificity. Control and known standard (1000, 500, 250, 125, 62.5, 31.25 & 15.6) were used for the study. Patients with inhibin B concentrations below detection limit i.e., 15 pg were assigned as undetectable. All the samples were tested in duplicate.
Microtitre plates were pre-coated with a monoclonal antibody to the beta-B subunit of inhibin. Samples (including control & standard) were incubated in the wells so that the antigen binds to the immobilized antibody via its βB subunit. Following washing a detection antibody was added. This was a monoclonal antibody specific for α subunit of inhibin coupled to alkaline phosphatase. Any unreacted material is then removed by washing before detection of alkaline phosphatase using a sensitive amplified substrate. This resulted in a red reaction product with color intensity proportionate to the concentration of dimeric inhibin B present in<


The mean (±SD) age and plasma levels of FSH & inhibin B of group A, B & C are shown in (Table/Fig 2). The age difference was not significant statistically (p = 0.95) between group A & C. Plasma FSH level was elevated in group A & B in contrast to group C women (P < 0.0001). Women in group A & B had undetectable (≤15 pg/ml) level of serum inhibin B whereas the mean level in group C was 51.8pg/ml. The difference was statistically significant (p <0.0001). Level of inhibin B in group A was no different than in group B.
Plasma FSH of group A/B and group C was inversely correlated with plasma inhibin B. There was not a single case with normal FSH/inhibin B in group A & B however there were five case in group A with non-menopausal (<40miu/ml) FSH. All had history of exogenous estrogen intake preceding 3 months of blood sampling (Table/Fig 3).
Interphase FISH with chromosome X centromeric probe was carried out in all cases of POF (group A). All excepting 2 were disomic for chromosome X (normal). Two cases of chromosome X mosaicism (XX/XXX) were detected however, frequency of trisomic (XXX) cell line was below 10%.


In current society, the desire of women to reproduce in later years leads to an increase incidence of infertility. Infertility workup and treatment is frequently time consuming, expensive and unsuccessful leading to economical & psychosocial difficulties. Hence, evaluation of ovarian status to identify women who has a chance of becoming pregnant before initiating expensive treatment becomes more important for proper prediction & counseling. This may decrease anxiety as well as marital disharmony. Ovarian status can be screened using various tests. FSH, estradiol, ovarian volume, antral follicle count, ovarian biopsy, clomiphene citrate challenge test or gonadotropin analogue stimulation test are commonly used. The clomiphene citrate challenge test is widely accepted method of testing ovarian reserve (18),(19),(20),(21), however, it requires few days to perform and multiple blood sampling. The gonadotropins analogue stimulation test is based on concentrations of FSH, estradiol and LH before and after GnRH analogue administration (22). Its limitations are expense, need for injections and repeated blood samplings in addition to limited ability to differentiate normal from reduced ovarian reserve (23). Hence there is a need for alternative simple test. Simple tests for ovarian reserve are ultrasonography (10),(24),(25) and FSH (11). However, they have their diagnostic limitations as ovulation may be seen in premature ovarian failuredespite high FSH (4) and subjectiveness of ovarian ultrasonography. Ovarian biopsy (26),(27) although reliable is invasive procedure. Hence there is a clear need for noninvasive, direct and precise marker of ovarian reserve. The identification of a parameter that can discriminate between complete absence of germ cells in the ovary and less severe disturbances of ova production would be of considerable prognostic value for assessment of female infertility. Inhibin B & AMH, which are direct product of the granulosa cells, may be more accurate in this regard. Both AMH and inhibin B are produced by the granulosa cells of preantral and small antral follicles of ovary during early folliculogenesis. Since the number of ovarian follicles declines with increasing age, AMH & inhibin B levels may be used as a direct & precise marker for ovarian ageing. Reports claim that inhibin B (2),(7),(8),(12),(28),(29),(30) and antimullerian hormone (10),(31),(32) are better markers of ovarian function.

This study was aimed to find out role of Inhibin B in assessment of gonadal function in premature ovarian failure women and compared with FSH. This cohort of patient was after exclusion of those suffering from overt/diagnosed medical disease or on medication. None of POF cases resumed spontaneous menstruation or conceived in one year (some up to 2 years)follow-up. The FSH rise in older women has been well documented (33),(34) and commonly utilized test for ovarian reserve. FSH level of ≥40miu/ml in two occasions at interval of 1 or more month/s is indicative of ovarian failure (35). Serum FSH levels was high in all POF & menopausal women in our study however there were 5 cases of POF with below menopausal (40miu/ml) FSH. Previous studies demonstrated that POF women with amenorrhea for less than 3 months and low


We conclude that undetectable inhibin B and high FSH constitute markers of ovarian failure and measurements can be a useful non-invasive one step tool for management as well as counseling of POF women who are seeking infertility treatment.

Conflict of Interest: None declared

Key Message

Inhibin B is a better predictor for ovarian function than FSH


The authors thank Indian Council of Medical Research, India for financial supported through the research grant for “Role of Cytokines on Premature Ovarian Failure.”


Burger HG. Clinical utility of inhibin measurements. J Clin Endocrinol Metab 1993; 76: 1391–1396.
Luisi S, Palumbo M, Calonaci G, De Leo V, Razzi S, Inaudi P, et al. Serum inhibin B correlates with successful ovulation in infertile women. J Assist Reprod Genet 2003; 20: 241-247.
Chada M, Prusa R, Bronsky J, Pechova M, Kotaska K, Lisa L. Inhibin B, follicle stimulating hormone, luteinizing hormone, and estradiol and their relationship to the regulation of follicle development in girls during childhood and puberty. Physiol Res 2003; 52: 341-346.
Welt CK, Hall JE, Adams JM, Taylor AE. Relationship of estradiol and inhibin to the follicle-stimulating hormone variability in hypergonadotropic hypogonadism or premature ovarian failure. J Clin Endocrinol Metab 2005; 90: 826-830.
Klein NA, Houmard BS, Hansen KR, Woodruff TK, Sluss PM, Bremner WJ, et al. Age-related analysis of inhibin A, inhibin B, and activin a relative to the intercycle monotropic follicle-stimulating hormone rise in normal ovulatory women. J Clin Endocrinol Metab 2004; 89: 2977-2981.
Luisi S, Florio P, Reis FM, Petraglia F. Inhibins in female and male reproductive physiology: role in gametogenesis, conception, implantation and early pregnancy. Hum Reprod Update 2005; 11: 123-135.
Tayyab M, Samiullah S, Ditta A, Jaffery G. Significance of serum inhibin in female infertility. J Ayub Med Coll Abbottabad 2001; 13: 24-25.
Ficicioglu C, Kutlu T, Demirbasoglu S, Mulayim B. The role of inhibin B as a basal determinant of ovarian reserve. Gynecol Endocrinol 2003; 17: 287-293.
Danforth DR, Arbogast LK, Mroueh J, Kim MH, Kennard EA, Seifer DB, et al. Dimeric inhibin: a direct marker of ovarian aging. Fertil Steril 1998; 70: 119-123.
van Rooij IA, Broekmans FJ, Scheffer GJ, Looman CW, Habbema JD, de Jong FH, et al. Serum antimullerian hormone levels best reflect the reproductive decline with age in normal women with proven fertility: a longitudinal study. Fertil Steril 2005; 83: 979-987.
Tinkanen H, Blauer M, Laippala P, Tuohimaa P, Kujansuu E. Correlation between serum inhibin B and other indicators of the ovarian function. Eur J Obstet Gynecol Reprod Biol 2001; 94: 109-113.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2017: 134.54
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • Embase
  • EBSCOhost
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)