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MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
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Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

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Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


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Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
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Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2011 | Month : February | Volume : 5 | Issue : 1 | Page : 78 - 81 Full Version

A Study On The Detection Of Micrometastases In The Cervical Lymph Nodes Of Oral Squamous Cell Carcinomas By Serial Sectioning


Published: February 1, 2011 | DOI: https://doi.org/10.7860/JCDR/2011/.1145
A RAVI PRAKASH¹, G S KUMAR², PUSHPARAJA SHETTY³

¹Department of Oral and maxillofacial pathology, G Pulla Reddy Dental College and Hospital, Kurnool, Andhra Pradesh, ² MDS, Department of Oral and maxillofacial pathology, KSR dental college, Tiruchengode, Tamilnadu, ³ MDS, Department of Oral and maxillofacial pathology, A B Shetty Dental College, Mangalore, Karnataka

Correspondence Address :
A Ravi Prakash, MDS
Department of Oral and maxillofacial pathology,
G Pulla Reddy Dental College and Hospital,
Nandhyal Road,
Kurnool - 518002,
Andhra Pradesh, India.
E-mail: drravi17@yahoo.com
Mobile No. +919448457595

Abstract

Background: Metastatic deposits in the regional lymph nodes have always been a subject of great interest in oncology. There is a need for the detection of micrometastases in the lymph nodes in oral carcinomas by serial sectioning, so that any missed tumour cells in the routine single sectioning technique could be detected by this. The present study was aimed at the detection of micrometastases in the cervical lymph nodes in oral squamous cell carcinomas- by serial sectioning.Materials and Methods: This study was done on 16 cases with 119 lymph nodes which measured 1cm or less in greatest diameter, by a single section routine method and this was compared with serial sectioning at 100µm intervals. All the 2269 sections were stained with the routine Hematoxylin and Eosin staining for the detection of micrometastases.Results: The detection of micrometastases by the serial sectioning method was 2.03%. The percentage was the same for both the one section and the serial sectioning methods.Conclusion: The serial sectioning of the lymph nodes did not reveal any other detection than that was revealed by the one section method. But definitely, the serial sectioning method appears to be the best feasible method to evaluate micrometastases.
Key words: Carcinoma, Lymph nodes, Micrometastases

Keywords

Carcinoma, Lymph nodes, Micrometastases

Metastasis, the spread of tumours, is one of the important characteristics of malignancy. Regional metastasis to the lymph nodes, which has a definite bearing on prognosis, is a widely debated topic and controversies exist with regards to its assessment and the line of treatment in oral squamous cell carcinomas. Regional metastasis to the cervical lymph nodes also reflects this confusing and conflicting scenario.

Accessing the lymph nodes in the absence of clinical enlargement is challenging. Occult metastases, which is otherwise known as micrometastases, are the microscopic foci of the metastasis in the nodes, which do not clinically show detectable enlargement. Specific limitations in the histological evaluation of nodes are dictated by the variables of the lymph nodes, the size of the metastatic lesion, as well as the method and number of the sections taken from the node.

Studies on micrometastasis have been done in gastric cancers (1), breast cancers (2), colorectal cancers (3) and in the carcinomas of other sites and their results have shown that the detection of micrometastasis has a significant difference in the recurrence and survival rate. Very few published data are present on the detection of micrometastasis in the cervical lymph nodes of oral squamous cell carcinomas (4) by serial sectioning.

This study was planned to evaluate the micrometastases in the cervical lymph nodes, which were equal to or smaller than 1cm, by serial sectioning of 100µm and to compare them with those which were detected by the routine one section technique in both node positive and node negative cases of oral squamous cell carcinomas.

Material and Methods

This study involved the retrieval of the cervical lymph nodes from formalin fixed, post operative specimens of the oral squamous cell carcinomas of 16 patients.

Lymph nodes which are equal to/or less than 1cm at their greatest diameter, were taken from the cervical region of Radical Neck Dissection (RND) specimens with the diagnosis of oral squamous cell carcinoma of different histological grades. Both node negative and node positive cases were considered for the study. No levels of the lymph nodes were considered for the study, as they were taken from the archives of the department.

The present study was carried out by harvesting a total number of 119 lymph nodes from the RND specimens of 16 patients, in which 3 were node positive and the other 13 were node negative cases. Each of these lymph nodes which were obtained, were evaluated both by the routine one section method and by serial sectioning at every 100µm and a total of 2269 sections were evaluated. The usefulness of serial sectioning in detecting micrometastases, when compared to the one section method, was evaluated. Each lymph node was cut at its greatest diameter.

The two halves of the lymph node were processed routinely and they were embedded in paraffin wax separately. The lymph nodes were then sectioned for obtaining single sections and then the whole lymph node was cut at every 100µm till it was exhausted. All the sections were stained by the routine H and E staining and they were checked for micrometastases by oral pathologists.

Results

(Table/Fig 1): The ages ranged from the third to the seventh decade. Out of 16 patients in the study, 5 were females and 11 were males. Seven were of the well differentiated, eight were of the moderately to well differentiated and one was of the moderately differentiated pathological types (grades?).(Table/Fig 2): Out of the 119 lymph nodes which were examined, 20 were of 0.6cm and 173 sections were made from it, 19 were of 0.7cm and 395sections were made from it and 42 were of 1.0cm and 850 sections were made from it.Micrometastases was detected in only two nodes of size- 1cm of a node negative case by the one section method and in the same case, by serial sectioning. No other lymph nodes by the one section method or by serial sectioning proved to be positive for micrometastases.

When comparison was made between the one section method and the serial sectioning method, identical results in detecting micrometastases were observed in both the methods, i.e. 2 positive lymph nodes out of 119 lymph nodes (2.03%)

Discussion

Metastatic deposits in the regional lymph nodes have always been a subject of great interest in oncology. The term ‘micrometastases’ itself has been defined by different authors differently. Black et al (5) defined micrometastases as tumours occupying <20% of the sectioned area. De mascarel et al (3)defined micrometastases as metastatic deposits which measured less than 0.5mm in diameter. Tumour deposits within the lymph nodes were classified and staged according to the revised guidelines which were set by the International Union against Cancer (UICC). According to this classification system, the metastases measuring 0.2mm to 0.2cm were considered as micrometastases. In our study, we considered the 0.2mm – 0.2cm deposits of malignant cells in the lymph nodes as micrometastases. The isolated tumour cells (ITC) are the individual malignant cells which are seen in lymph nodes, which can be detected only by immunohistochemistry.(5)

Many authors have carried out studies on the evaluation of micrometastases of the lymph nodes in gastric cancers (Mahera Y et al (6), Ishii et al (7)), colorectal carcinomas (Davidson BR et al(8), Isozaki et al (9), breast cancers (Tsuchiya et al (10), Harry D Bear et al (11), Hainsworth et al (12)), cervical cancers (Okamoto et al (13)), vulval cancers (Narayansingh G V et al (14)) and oesophageal carcinomas (Natsugoe S et al (15), Stephen McGrath et al (16)). There are very few published articles on oral squamous cell carcinomas (Joes E et al (17).

Different methods were employed to detect micrometastases, in which serial sectioning was the most commonly used method. Many controversies exist on whether serial sectioning is useful or not.

In breast cancers, Nasser et al (18) employed serial sectioning at 150µm intervals and he detected a 31% significance rate. M C Guckin et al (19) did serial sectioning at 100µm intervals and detected a 25% significance rate. Thus, it can be seen that the detection rate varied from 9 – 33%, and that it had no correlation with the serial sectioning intervals. The present study detected micrometastases in only two nodes out of 119 nodes of the 16 cases, of which 96 were from node negative and 16 were from node positive cases.

The detection rate was 2.03% and when compared to the one section method, the results were identical. The rate of detection of the micrometastases in breast cancer varied from 9 – 33%, and even recent studies by Jose E et al (17) 2003, showed that the detection of micrometastases by serial sectioning in oral cancer is beneficial. However, there are no studies on oral squamous call carcinomas, where they selected only lymph nodes of size less than 1cm and did serial sectioning at 100µm intervals. The lesser value of detection could be attributed to a limited sample size of 119 lymph nodes of 16 cases.

Other researchers used a much larger sample (88 – 3349). The sample size had to be curtailed because of the constraint of time and due to the selection of lymph nodes of size 1cm or lesser than that. Other studies had included all the lymph nodes of all sizes and so, the probability of detecting metastases in larger lymph nodes was higher. The selection of lymph nodes of size 1cm or less in this study had clinical importance, as they usually escape clinical detection. Therefore, the evaluation was restricted to lymph nodes which were sized 1cm or less, so that the detection of micrometastases in these could be of clinical significance. Due to limited positivity and identical results, no definite comment can be made about the advantages of serial sectioning over the one section method. However, the opinions vary between some researchers, (Nasser et al (18), Jose E et al (17)) claiming the superiority of serial sectioning over the one section method, while others (Wilkinson et al (20), Hartviet et al (21), De Mascarel et al (3)) claim that it is not so. In the present study, we faced some problems in the detection of the malignant cells within the parenchyma of the lymph node. Great difficulty was experienced in differentiating a single cell or a group of malignant cells which invaded the lymph nodes in the H and E sections, because the endothelial cells and the histiocytes closely resembled the malignant squamous cells (Table/Fig 4)(Table/Fig 5)(Table/Fig 6). However, the comparison of these cells with the endothelial cells in forming a lumen elsewhere in the same section and the absence of hyperchromatism, great variation in size, shape and keratin formation, helped to differentiate these from the malignant squamous cells Trivedi et al(23)(24) (25)(26)(Table/Fig 6)

This difficulty was reported by Nasser et al (18), where there was confusion between the epithelial cells, the benign nevus cell nests, the sinus histiocytes laden with keratinous debris and the keratin positive reticulum cells of the lymph nodes. Therefore, it might be possible that in this study, a few foci of malignant squamous cells may have been missed, though such sections were examined and discussed with qualified oral pathologists for a consensus.

The impact of micrometastases on prognosis has been an issue of great debate. It is a common experience that node negative patients come back with metastases after few years of therapy and that in these patients, the cause is often undiagnosed, untreated micrometastases of the lymph nodes (Fisher et al (22), Rosen P P et al (23))

Immunohistochemistry may help in differentiating the endothelial cells from the epithelial cells and also, the smaller foci of epithelium in the back ground of the highly cellular lymphocytes. However, the use of immunohistochemisty was not resorted in our study. The present study was limited to the evaluation of lymph nodes by serial sectioning and the routine H and E staining.

Conclusion

The present study did not reveal much change between the 1 section technique and the serial section method. As metastases are a significant feature in the treatment and prognosis of tumours, we suggest that serial sectioning is definitely one of the best feasible methods to evaluate micrometastases, and it is advised to evaluate the micrometastases by serial sectioning for at least suspicious, small lymph nodes, if all the nodes were not excised.

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