Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
On May 11,2011

Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
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Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
On April 2011

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.

Dr. Anuradha
On Jan 2020

Important Notice

Original article / research
Year : 2011 | Month : October | Volume : 5 | Issue : 5 | Page : 929 - 931 Full Version

Pedigree Analysis and Cytogenetic Study in Vitiligo

Published: October 1, 2011 | DOI:
Archana Shekokar, Ramakrishna Ghubde

MBBS MS, Professor Dept of Anatomy, SKN Pune, India. MBBS MD, Chest Medicine, Fellowship in Critical Care Medicine, SRM Medical College Chennai, India.

Correspondence Address :
Archana Shekokar, SKN Narhe,
Pune, Maharastra,
Phone: 9881014985


A total 300 cases of vitiligo with family history & cytogenetic study in patients having positive family history male to female ratio 1:1. Vitiligo begins in the younger age groups, symmetrical lesions in 50% cases, 72 cases gave definite positive family history & transmitted by autosomal dominant characteristic.


Vitiligo, Multilocus recessivity, Segregation analysis

Leucoderma is a general term applied to decreased melanin pigmentation of skin. Vitiligo is the commonest type of leucoderma. Daniel Turner (1714) was the first British dermatologist, who recognized vitiligo. Vitiligo is an idiopathic, hypo melanositic, dermatological disorder characterized by pale milky white macules that tend to become progressive over time. It is not present at birth but develops later in life (1), (2), (3).

The disease is characterized by loss of normal colour of the skin in patches, resulting in various degrees of cosmetic disfigurement. It may cause a severe sense of humiliation, worry, anxiety & represent a social obstacle throughout life. The affected patient prefers seclusion & abstains from appearing in public, lest he should be looked upon as having a contagious disease (4).

The oldest information concerning it comes from Pharonic Medicine in the Ebers papyrus. The Indian sacred book Atharva Veda mentions a disease known as “shweta-kustha” (5). In Arabic language bohak, bahak, baras are Arabic names for vitiligo. The term vitiligo, derived from the Latin word ‘vitelius’, means calf. It was first used by the Roman physician Celsus.

Genetic control of pigmentation in mammalian systems is complex. At least 147 genes at 53 different loci affect coat colour in mice. Inherited pigmentary defects in humans are known to involve mutations in genes which control various steps in the pigmentary process. In general, mutations which interfere with the development & migration of melanoblasts to peripheral sites are frequently associated clinically with localized hypopigmentation & deafness (6), (7), (8).

While those mutations which interfere with melanosome formation and the synthesis of melanin result in defects with the general clinical characteristics of oculocutaneous albinism.

• Family study [pedigree analyses] of vitiligo patients. • Cytogenetic study of patients having positive family history of vitiligo. • To find out whether or not any chromosomal abnormality exists in vitiligo patients.

Material and Methods

The present study, carried out in Department of Anatomy, GMC Nagpur, comprises of pedigree analysis [family study] & cytogenetic study of 300 cases of vitiligo patients from Skin OPD, clinically diagnosed cases of vitiligo were selected. Patients were studied with age, sex, geographic area, occupation, education status, food habits includes alcohol intake, family history up to 3 generations, history of allergy, size, shape, symmetry, distribution, pattern & colour of borders in vitiligo (9), (10), (11).

Chromosomal studies were carried out on blood lymphocyte culture. Peripheral blood for culture was drawn from the median cubital vein under complete aseptic conditions, in a heparinized syringe. Then 3-4 drops of blood was dropped into culture bottles containing RPMI 1640 medium. Culture bottles were incubated at 37oC for 72 hrs with stopper tightly closed. To arrest mitosis colchicine was added to medium 2 to 3 hrs before harvesting. The cultures were centrifuged twice at 1000 RPM for 5 minutes. Phytohaemagglutin was added to swell the cells & they were fixed in alcohol at room temp for 30 minutes. The supernatant was discarded completely without disturbing the cells at the bottom. Then 2-3 drops of cell suspension were dropped on wet, ice cold, grease free slides from a distance, to facilitate spreading. Further the slides were stained by Giemsa stain. Karyotyping of the slides was done using photokaryotyping. A camera was attached to the microscope. Photographs of the metaphase spread were taken. From the photograph of each metaphase spread, the chromosomes were cut out & aligned according to centromere position. In this manner the karyotypes were prepared for all 50 cases (7), (8).



The following observations were found in present study.

In our study, vitiligo appears in younger age group. The male: female ratio was 1:1 (Table/Fig 1)(Table/Fig 2)(Table/Fig 3)(Table/Fig 4)(Table/Fig 5) (Table/Fig 6)(Table/Fig 7).

The commonest size of vitiligo lesions ranged from 2 to 5 cm in 200 cases, and symmetrical lesions were observed in 50% cases (Table/Fig 8)(Table/Fig 9).
72 cases [24%] out of 300 gave a positive family history (Table/Fig 2).

Out of 72 cases, 10 [3.33%] had more than one vitiligenous blood relation. In the remaining 62 cases, [20.66%] the patient had only one relation suffering from the disease. Of these, 22 [7.33%] had a vitiligenous mother, 18 [6%] had a vitiligenous father, 10 [3.33%] had a vitiligenous sister, 11 [3.66%] had a vitiligenous son/daughter, 3 [1%] had a vitiligenous uncle /aunt & 3 [1%] had vitiligenous cousins (6), (9), (10).

Several authors reported similar findings.

Status of disease: In 227 cases [75.66%] the disease was progressive & remained stationary in 73 cases [24.33%].

Chromosomal analysis was carried out in the 70 patients who had positive family history. Metaphase spreads could be studied in 50 cases [71.42%] since cultures failed in 20 cases [28.57%]. The karyotype was normal; there were no structural & numerical abnormalities (11) (Table/Fig 10).


Vitiligo presents itself with onset at an early age. The lesions tend to be small in size yet progressive. Vitiligo is more evident in early ages of males (upto 30 years of age) than females. Vitiligo follows a polygenic, multifactorial inheritance pattern. However, none of the karyotypes presented structural or numerical abnormalities. Significant family inheritance presents itself as an appreciable factor for this disease. Chromosomal changes eg metaphase are evident in half of cases.


Arora PN, Behl PN, Bhatia RK: Vitiligo: A series of 403 cases. Ind J Dermatology: 1993; 38: 2-6.
Nath SK, Mujumder PP, Nordlund JJ: Genetic epidemiology of vitiligo: multilocus recessivity cross validated. Am J Hum Genet 1994; 55: 981-90.
Mujumder PP, Nordlund JJ, Nath SK: Pattern of familial aggregation of vitiligo. Arch Dermatol 1993; 129: 994-98.
Le Poole IC, Das PK, Vandan Wijngaard RMJGJ, Bas JD, Westerhof W: Review of the epthiopathomechanism of vitiligo: a convergence theory. Exp Dermatol 1993;2:145-53.
Whitney WD: Atharva–veda samhita. Harvard oriental series, Cambridge. 1905;5.
Lander ES, schork: Genetic dissection of complex traits. Science 1994; 265: 2037-48.
Lison M, Kornburt B, Feinstein A, Hiss Y, Bolchish H, Godman RM: Vitiligo: Premature greying & distinct facial appearance: A new genetic syndrome in 3 siblings. Am J Med Genetics 1961; 9: 351-57.
Thompson & Thompson: Chromosomal aberrations. Philadelphia 1980;142.
Koranne RV, Sehgal VN, Sachdeva KG: Clinical profile of vitiligo in north India. Ind J Derm Venereal Laprol 1986; 52: 81-82.
Nordlund JJ, Halder RM, Grime SP: Management of vitiligo. Dermatol Clin 1993; 11: 27-33.
Boisseau Garvsaud AM, Garsaud P, Cales-Quistd, Helenon R, Quenehevve C, Charle- Sainte-Claive R et al.: Epidemiology of vitiligo in the French West Indies (Isle of Martinique). Int J Dermatol 2000; 39: 18-20.

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