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Aug 2018




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Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
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Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Dentistry
Year : 2012 | Month : May | Volume : 6 | Issue : 3 | Page : 517 - 520

Diabetes and Oral Changes: The Tryptophan Metabolism Link?

Rishabh Kapila, Kikeri Seetaramaih Nagesh, Asha Iyengar, Divyalakshmi

1. Senior Lecturer, Department of Oral Medicine and Radiology, Guru Nanak Dev Dental College and Hospital, Sunam, Punjab, India 2. Professor, Head and Principal, Department of Oral Medicine and Radiology, D.A.P.M.R.V. Dental College and Hospital, Bangalore, Karnataka, India. 3. Professor, Department of Oral Medicine and Radiology, D.A.P.M.R.V.Dental College and Hospital, Bangalore, Karnataka, India. 4. Reader, Department of Oral Medicine and Radiology, D.A.P.M.R.V.Dental College and Hospital, Bangalore, Karnataka, India.

Correspondence Address :
Rishabh Kapila
House Number 110, Sector 12-A
Panchkula, Haryana - 134109, India.
Phone: +91-925663774
E-mail: rishabh_kapila@rediffmail.com

Abstract

Objectives: The objective of this study is to determine whether the severity of periodontal disease is associated with an increase in the levels of tryptophan and its metabolites in the saliva of type 2 diabetic patients.

Materials and Methods: 40 subjects were selected for this study. Each subject of the study was subjected to a detailed case history and intraoral examination. Then, the glycosylated haemoglobin estimation, the CPI scoring and the assessment of the salivary tryptophan levels and the levels of its metabolite were done. The data which was obtained was subjected to statistical analysis.

Results: The mean salivary tryptophan metabolites were higher in diabetic individuals than non-diabetic individuals. An increase in the CPI score was associated with an increase in the levels of the salivary tryptophan metabolites.

Conclusion: A relationship exists between the severity of periodontal disease and the levels of salivary tryptophan and its metabolites in type 2 diabetic individuals. This study may add an insight into an alternate pathway for the development of periodontal disease in the type 2 Diabetes mellitus individuals and kynurenergic agents, which may act as new treatment modalities for such cases.

Keywords

Diabetes; Periodontal Disease; Tryptophan

Introduction
Diabetes mellitus is a metabolic disease which is characterized by the dysregulation of carbohydrate, protein and lipid metabolism. The primary feature of this disorder is the elevation in the blood glucose levels (hyperglycaemia), which results from either a defect in the insulin secretion from the pancreas or a change in insulin action, or both (1). Diabetes could result, in part, in the activation of the tryptophan metabolism. This reduces the plasma tryptophan levels and elevates the kynurenine metabolite levels. Several products of the kynurenine pathway have anti insulin action (2). Tryptophan metabolites, via the kynurenine pathway, play an important role in several fundamental biological processes, including neuronal excitability, antioxidant status, ultravioletprotection, cell growth and cell division (2). The salivary kynurenine derivatives are also implicated in the onset and the development of periodontal disease in humans (3). Diabetic patients are more susceptible to gingivitis and periodontitis than the healthy subjects. These diseases are commonly considered to be the oral complications of diabetes (4). The oral conditions that are seen in individuals with diabetes may include a burning mouth, an altered wound healing and an increased incidence of infection. Enlargement of the parotid glands and xerostomia can also occur (5). Thus, there is a possibility that the kynurenine pathway derivatives which are present in saliva may play a role in the onset and the development of periodontal disease in diabetic patients (2).

Material and Methods

An informed signed consent was obtained from all the subjects prior to their inclusion in the study. All the procedures were followed in accordance with the ethical standards and with the last update of the Helsinki Declaration.A total number of 40 subjects were included in the study. The subjects who were selected included 22 males and 18 females of the age group 40 years and above. These subjects were then divided into four groups: Group A: 10 patients with normal blood glucose levels. (Control group); Group B: 10 patients of confirmed type 2 diabetes with a glycosylated haemoglobin assay of < 7%; Group C: 10 patients of confirmed type 2 diabetes with a glycosylated haemoglobin assay between 7%–8%; and Group D: 10 patients of confirmed type 2 diabetes with a glycosylated haemoglobin assay of > 8%.

All the individuals were subjected to a thorough general and oral examination including their periodontal status by using the Community Periodontal Index (CPI). 2ml of saliva was then collected and sent for the estimation of the levels of the salivary tryptophan metabolites by the High Performance Liquid Chromatography (HPLC) method. The general examination of the subjects in both the non-diabetic and the diabetic groups included findings like retinopathy (vision changes, blindness), neuropathy, nephropathy, trophic ulcer and altered wound healing. In the intra-oral examination, the tongue and the buccal and the labial mucosa were examined for pallor and ulcerative lesions. The patients were questioned about any taste alterations or burning sensations. The salivary glands were assessed for any enlargement and for xerostomia or ptyalism. The history of dryness or stickiness in the mouth was noted. Then, the orifices of all the major salivary gland ducts were dried with sterile, dry gauze and the flow and the consistency of saliva was checked by milking the ducts.

Saliva Sampling
The patient was made to sit comfortably on the dental chair. He was then asked to wash his mouth with MilliQ water (prepared byreverse osmosis) for 10 minutes. Then, 2 ml of non-stimulated, mixed saliva was collected from all the subjects (non-diabetic and diabetic) in Eppendorf tubes. The salivary samples were immediately treated with 2 M perchloric acid (HClO4) and were transferred to centrifuge tubes. After 15 minutes of incubation with the acid at 4ÂşC, the samples were centrifuged for 30 minutes at 12000 x g and the supernatant was collected for the measurement of the concentration of tryptophan and its metabolites by High Perfomance Liquid Chromatography (HPLC).

Evaluation of the Salivary Tryptophan Metabolites
The concentrations of tryptophan, anthranilic acid and kynurenic acid were determined by high performance liquid chromatography by using the supernatant which was collected after the saliva sampling. The reversed−phase HPLC system consisted of a Waters Spherisorb S3 ODS2 150 Ă— 2.1 mm column, an HP 1050 series pump and a Rheodyne injection valve which was fitted with a sample loop (5 μl). The column effluent was monitored by using a programmable fluorescence detector, HP 1046A. The optimized conditions were determined by recording the fluorescence spectra by using a stop-flow technique. The excitation and emission wavelengths were set to 254/404 nm for tryptophan and kynurenic acid and 320/420 nm for anthranilic acid respectively. The output of the detector was connected to a ChemStation and to a computer which had the software for carrying out the measurements. The mobile phase consisted of 50 mM acetic acid and 0.25 M zinc acetate (pH –4.9) containing 1% of acetonitrile and it was pumped at a flow−rate of 0.18 ml/min. The chromatography was carried out at 21oC.

Chromatograms were obtained for tryptophan, kynurenic acid and anthranilic acid. These chromatograms were later used for estimating the peak area for the quantification of the data by using a data processor system. The width at half-height was obtained by multiplying the peak width at half-height by the peak height, to determine the area and the results were expressed in nanomoles per litre. These results indicated the salivary levels of tryptophan, kynurenic acid and anthranilic acid. The Excel and SPSS (SPSS Inc, Chicago) software packages were used for data entry and analysis by using ANOVA and the Bonferroni test. p-values of < 0.05 were regarded as statistically significant.

Results

A detailed history of the general symptoms of the individuals in the diabetic group revealed that 08 subjects (26.66 %) had vision changes and that 03 subjects (10 %) had peripheral neuropathy. A detailed history of the oral symptoms of the individuals in the diabetic group revealed that 7 subjects (23.33 %) had burning mouth symptoms, 4 subjects (13.33 %) had dysguesia, 3 subjects (10 %) had sialadenosis, 2 subjects (6.66%) had xerostomia and that 1 subject (3.33%) had oral ulcers. In the non-diabetic group, none of the above mentioned general and oral manifestations were present in the subjects. The Community Periodontal Index (CPI) score was calculated for both the non-diabetic group and the diabetic group to determine the periodontal status. The mean CPI score was greater in the poorly controlled diabetic group (3.51 ± 0.53), followed by the moderately controlled group (2.67 ± 0.47), the well controlled group (2.67 ± 0.47)and finally, the non-diabetic group (1.89 ± 0.32). The results showed that the mean CPI values increased with the severity of diabetes and this difference was found to be statistically significant (p<0.001). However, no statistically significant difference was observed between the non-diabetic group and the well controlled diabetic group with respect to the mean CPI (p>0.05) (Table/Fig 1).

The mean salivary tryptophan and kynurenic acid levels within the diabetic study group and on comparison with the non-diabetic group were found to be statistically significant (p< 0.001) (Table/Fig 2) & (Table/Fig 3). A statistically significant difference was found between the mean salivary anthranilic acid levels of the poorly controlled diabetic group and the well controlled diabetic group as well as the non-diabetic group (p<0.001). However, the mean difference was not significant between the poorly controlled and the moderately controlled diabetic groups (p>0.05) and; between the well controlled diabetic and the control groups (p>0.05) (Table/Fig 4). In order to find out whether a correlation existed between the Community Periodontal Index scores and the salivary tryptophan metabolites i.e. the salivary tryptophan, kynurenic acid and anthranilic acid levels, the Pearson’s correlation test was applied. The results of the Pearson’s correlation test suggested that with an increase in the severity of periodontal disease, the salivary tryptophan, kynurenic acid and anthranilic acid levels also increased and vice versa (Table/Fig 5).

Discussion

Diabetes mellitus is a clinically and genetically heterogeneous group of metabolic disorders which are manifested by abnormally high levels of glucose in the blood (6).The six main complications which are associated with diabetes mellitus are retinopathy, nephropathy, neuropathy, macrovascular disease (cardiovascular disease, cerebrovascular disease, peripheral vascular disease), altered wound healing and periodontal disease. Diabetes mellitus and periodontal disease are two common chronic diseases that have long been considered to be biologically linked (7). Tryptophan, which is an essential amino acid, is required for the biosynthesis of proteins and it is the precursor for several biological compounds (8). The role of tryptophan and its metabolites in the oral physiology and pathology have not been studied as yet. Tryptophan was found in significant amounts in the saliva of diabetic individuals in some studies, particularly tryptophan metabolites like kynurenine and anthranilic acid (2). In a study which was conducted in a Chinese population, results which were similar to those of our study were noted, with an increase in the prevalence of the vision changes (9). Studies have shown an association between diabetes and oral changes. The significant oral changes which were observed in diabetics were hyposalivation which led to oral dryness, altered taste, burning mouth sensation, angular cheilitis, glossitis, and stomatitis (10),(11). An association between diabetes and periodontal disease has long been looked into. Diabetes increases the likelihood of developing periodontal diseases to two- to five-fold (12). To find out any such association, the Community Periodontal Index (CPI) score was calculated for both the non-diabetic and the diabetic groups. The results which were obtained were in accordance to those of studies which were conducted to investigate the associations between glycaemic control and the severity of periodontal disease. It has been shown that the gingival and periodontal score was statistically higher in the diabetic group than in the non diabetic group (13). There was a strong associationbetween poorly controlled diabetes and severe periodontitis (14).(15),(16),(17),(18),(19). Moreover, a study had already indicated that all the type 2 diabetic patients should be informed about their increased risk for periodontal disease (20),(21). Tryptophan, an essential amino acid, is metabolized via the kynurenine pathway to form metabolites like kynurenine, kynurenic acid and anthranilic acid (22). Recently, tryptophan and its metabolites such as kynurenic acid and anthranilic acid, have been implicated in a number of disorders including diabetes and periodontal disease. The results of our study were in accordance to those of a study which was done to estimate certain tryptophan derivatives in the saliva of patients with diabetes type 2. The increased concentrations of kynurenine and kynurenic acid in patients with diabetes and hypertension as compared to those in the healthy volunteers suggested an altered metabolism of tryptophan in these diseases (2).

A correlation between the community periodontal index and salivary tryptophan and its metabolites suggested that with an increase in519the community periodontal index score (suggesting the severity of the periodontal disease), the levels of tryptophan and its metabolites also increased. Moreover, an increase in the salivary tryptophan metabolites was also associated with an increase in the severity of periodontal disease. This correlation between diabetes mellitus, periodontal disease and the salivary tryptophan metabolite levels could be related to the impaired kynurenine pathway metabolism of tryptophan. There are so far no documented studies to the best of our knowledge, that correlate the severity of periodontal disease with the salivary tryptophan metabolites between non-diabetic and diabetic individuals and within the 3 diabetic groups.

A considerable number of pharmacological tools have recently been made available to probe the kynurenine pathway experimentally. Some of these kynurenergic agents have been envisioned to be of therapeutic value, especially in the treatment of diseases that are associated with the impaired kynurenine pathway metabolism (23).

Conclusion

The role of the metabolites of tryptophan via the kynurenine pathway has been speculated in type 2 diabetes due to its antiinsulin action. The salivary levels of tryptophan and its metabolites may have some bearing in the progress of the periodontal disease in diabetic individuals. Certain agents are available, that tap the kynurenine pathway i.e. kynurenergic agents and these can be envisioned to be of therapeutic value, especially in the treatment of diseases that are associated with the impaired kynurenine pathway metabolism. This may open new avenues for the management of the periodontal disease in the Diabetes mellitus type 2 cases.

Acknowledgement

My sincere thanks to Thejasvi TV for carrying out the statistical analysis and Dr. Guruprasad for helping in the liquid chromatography procedure.

References

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Martin GS, Glick M. Burket’s Oral Medicine, diagnosis and treatment. 10th edition. India: Elsevier: 2003; 563.
2.
Buczko P, Stokowska W, Gorska M, Kucharewicaz I. Pawlak D, Buczko W. Tryptophan metabolites via the kynurenine pathway in the saliva of diabetic patients. Dental Medical Problems 2006; 43 (1): 21-25.
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Buczko P, Cylwik D, Stokowska W. Metabolism of tryptophan metabolism via the kynurenine pathway in saliva. Postepy Hig Med Dosw. 2005; 59: 283-89.
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Matthews DC. The relationship between diabetes and periodontal disease. J Can Dent Assoc. 2002; 68 (3): 161-64.
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Martin GS, Glick M. Burket’s Oral Medicine, diagnosis and treatment. 10th edition. India: Elsevier: 2003; 570-71.
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Mealey BL, Ocampo GL. Diabetes mellitus and periodontal disease. Periodontology 2000; 44: 127-53.
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Taylor GW, Borgnakke WS. Periodontal disease: associations with diabetes, glycemic control and complications. Oral diseases 2008; 14: 191-203.
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Widner B, Werner ER, Schennach H, Wachter H, Fuchs D. Simultaneous measurement of serum tryptophan and kynurenine by HPLC. Clinical Chemistry. 1997; 43: 2424-26.
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Fermin CA, Paulo CM. The Periodontal Pocket. In: Carranza’s Clinical Periodontology. 10th ed. India: Mosby, 2007; 434-42.
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DOI and Others

DOI: JCDR/2012/3584:2075

Financial OR OTHER COMPETING INTERESTS:
None.


Date of Submission: Jul 27, 2011
Date of Peer Review: Oct 17, 2011
Date of Acceptance: Dec 22, 2011
Date of Publishing: May 01, 2012

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