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Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
Timely publication of journal: Publication of manuscripts and bringing out the issue in time is one of the positive aspects of JCDR and is possible with strong support team in terms of peer reviewers, proof reading, language check, computer operators, etc. This is one of the great reasons for authors to submit their work with JCDR. Another best part of JCDR is "Online first Publications" facilities available for the authors. This facility not only provides the prompt publications of the manuscripts but at the same time also early availability of the manuscripts for the readers.
Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
It is well said that "happy beginning is half done" and it fits perfectly with JCDR. It has grown considerably and I feel it has already grown up from its infancy to adolescence, achieving the status of standard online e-journal form Indian continent since its inception in Feb 2007. This had been made possible due to the efforts and the hard work put in it. The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. I must thank and congratulate Dr Hemant Jain, Editor-in-Chief JCDR and his team for their sincere efforts, dedication, and determination for making JCDR a fast growing journal.
Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."
Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011
Dr. Shankar P.R.
"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."
Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011 Anuradha
Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.
Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020
Original article / research
Full Version
Simple Stain For Helicobacter Pylori
Correspondence Address :
Dr. D. Vijaya
Professor& HOD Microbiology
AIMS, B.G.Nagara 571448
Karnataka, India.
Phone no; 94820 09120
E Mail; vijayadanand@rediffmail.com
Several methods are available for the detection of Helicobacter pylori in the gastric antral biopsies. Most of these are technically demanding, slow and expensive with varying sensitivity. Aim of the present study was to find out the prevalence of Helicobacter pylori in various gastroduodenal diseases and to evaluate the advantages of Loeffler’s methylene blue stain for crushed biopsy smear.
A total of 236 (male 176, female 60) randomly selected patients with endoscopically proved chronic duodenal ulcer (180), gastroduodenitis (42) and gastric ulcer (14) formed the study group. Four gastric antral biopsies were taken from each patient. Biopsies were subjected to histology, rapid urease test, Gram’s and Loeffler’s methylene blue (LMB) stains for crushed smears. Evidence of H. pylori infection was found in 62.71% considering histopathology as the gold standard. H. pylori was more common in males (65.90%) and in the age group of 31-40 years (77.50%). In duodenal ulcer, gastric ulcer and gastroduodenitis, 68.88%, 57.14% and 38.09% were positive for H. pylori respectively. A smear stained with LBM was found to be rapid, simple, reliable, and cost effective with good sensitivity and specificity for detecting H. pylori.
Helicobacter pylori, Loeffler’s methylene blue stain
Introduction
Helicobacter pylori is an important pathogen in humans causing chronic gastritis & playing a major role in the development of peptic ulcers & gastric cancer (1). The present study was undertaken to find out the presence of H.pylori in various gastroduodenal diseases & to evaluate the advantages of Loeffler’s methylene blue (LMB) stain for crushed biopsy smears. The study was conducted in the department of Microbiology and Gastroenterology of B and L.C. Hospitals Bangalore. The study period was from July 2000 to December 2003.
A total of 236 randomly selected, symptomatic patients (male 176, female 60) in the age group of 16-65 years, endoscopically diagnosed as duodenal ulcer (180), gastroduodenitis (42) and gastric ulcer (14) formed the study group. Ethical clearance from the institution and consent of patients was taken prior to endoscopy.
Four endoscopic antral biopsy specimens from each case were transported in vials containing normal saline, urease medium & 10% formalin. From the specimens in normal saline, two crushed smears were made and fixed with methanol.Of this one smear was stained by Gram’s using carbol fuchsin as counter stain (Table/Fig 1). 2nd smear was stained with LMB for 30 seconds (Table/Fig 2). Specimens in urease medium were incubated at 370C for 60 minute which turns pink if positive (2). Histopathology sections were stained by over night Giemsa stain,which is considered as the gold standard for the detection of H.pylori (Table/Fig 3).
Of the 236 cases studied 176 were males & 60 females. The age varied from 16-65 years. A total of 148 (116 males, 32 females) were positive for H.pylori infection, considering histopathology section stained by Giemsa as the gold standard.
In duodenal ulcer, gastric ulcer and gastroduodenitis, 68.88%, 57.14% and 38.09% were positive for H. pylori respectively.
Various techniques are available for detection of H.pylori, but the aim of modern research is to find a method, which is rapid, cost effective and accurately identifies the H. pylori. The sensitivity of the test depends on the number of bacteria present in the specimen. In experienced hands every test has good sensitivity & specificity (3).
Out of 236 cases 148 (62.71%) were positive and 88 (37.29%) negative for H.pylori. H.pylori was more common in males (65.90%) than females (53.66%). H.pylori positivity was found in all the age group (16-65 years), as exposure to H.pylori occurs early in India & is widespread (4). Maximum positivity was found in age group of 31-40 years (77.50%). In duodenal ulcer, gastric ulcer and gastroduodenitis, 68.88%, 57.14% and 38.09% were positive for H.pylori respectively. Nair D, Ayyagari in their studies have found similar prevalence of H.pylori in all three conditions mentioned (5),(6). This prevalence has not increased as seen from the report presented from this department in 1999 (7).
The rapid urease test has the advantage of being simple, inexpensive, rapid & positive in 60 minutes, as evidenced in other studies also (2),(8). False positives could occur in urease test due to its high sensitivity(100%).
Crushed smears stained with Gram’s showed sensitivity of 98%, specificity of 100% can provide rapid diagnosis. This is in correlation with the study of VanHorn (9). No false positive will be found in an experienced hand. False negative may be due to, organisms hidden in proteinaceous material.
H.pylori take up deep blue and mucus faint blue colour in LMB stain and stood very well against the background. Staining time was only one minute. Smears stained with LMB showed high sensitivity and specificity, is similar to the study of others (10),(11). LMB staining is technically simple, rapid, reliable, inexpensive, with high positive and negative predictive value ,hence found to be excellent stain for the detection of H.pylori as it can pick up a very light load of infection, compared to technically demanding, expensive, slow histopathology and/or culture method (10),(11).
Authors are of the opinion that smear stained with LMB has high specificity (95.45% )and PPV(97.36), will be sufficient for the detection of H.pylori in an ordinary set up.
ID: JCDR/2012/4038:0029
Date of Submission: Mar 28, 2012
Date of peer review: Apr 23, 2012
Date of acceptance: Apr 26, 2012
Date of Publishing: May 31, 2012
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