Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
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Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
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KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


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E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2012 | Month : April | Volume : 6 | Issue : 2 | Page : 211 - 214

H.Pylori Associated Gastritis

Dandin Archana S., Pawale Jayashree, Athanikar V.S.

1. Corresponding Author, 2. Associate Professor, SN Medical College Department of Pathlogy. S Nijalingappa Medical College, Bagalkot, India. 3. Professor, BLDEA’s shri BM Patil Medical college

Correspondence Address :
Archana S Dandin,
c/o S M Dandin, Extension Area.
Bagalkot-587101, Karnataka, India.
Phone no: 09448960186
Email: dandinhosp@yahoo.co.in

Abstract

Introduction: H.Pylori has been associated with various upper gastro-intestinal tract disorders including gastritis, peptic ulcer and gastric malignances. There is a paucity of literature regarding the study of morphological changes in H.Pylori associated gastritis, as H.Pylori colonized gastric mucosa is a distinct pathologic entity with a pathologic spectrum ranging from active chronic gastritis to erosions & frank ulcer.

Objective: The aim of this study was to know the morphological changes seen in gastric mucosa associated with H.Pylori. And also to find out prevalence of H.Pylori in patients undergoing upper gastro-intestinal endoscopic biopsies with gastritis in the centre.

Materials and Methods: A total of 100 patients who were clinically diagnosed as having acute or chronic gastritis were included for this study during the period December 1999 to December 2001. A detailed clinical history was taken as per the standard proforma. Then the patients were subjected for endoscopy. Five endoscopic biopsies were taken and processed for rapid urease test and histopathological examination.

Results: Out of 100 cases of endoscopically diagnosed gastritis, 48 cases were H.Pylori positive. In this 48 H.Pylori positive cases, 43 were positive by both rapid urease test (RUT) and histopathology. 3 cases were RUT positive but negative for H pylori by histopathology, and 5 cases were negative by RUT but histopathology showed presence of H.Pylori. Morphological changes specific for H.Pylori colonization and characteristic features are irregular surface epithelium, loss of apical mucin, cell dropout, formation of pits and microerosions. Out of 100 cases, 46 were RUT positive and 54 cases RUT negative. 48 were histology positive and 52 were histology negative. Statastically, no significant difference between urease test and histopathological demonstration of H.Pylori (p>0.05).

Conclusions: Prevalence of H.Pylori in the present study was 48% in patients undergoing upper gastro-intestinal endoscopic biopsies with gastritis in this centre. H.Pylori infection is associated with spectrum of histological changes in gastric mucosa, which in turn facilitates the identification of H.Pylori. RUT and histopathology are the 2 diagnostic methods which can be used with an equal importance for the detection of H.Pylori associated lesions.

Keywords

H. Pylori associated gastritis, Endoscopic biopsies, RUT, Giemsa staining, Histopathological features

INTRODUCTION
The study of gastric bacteriology remained neglected for a long period. It has gained importance since the isolation of H.Pylori from gastric biopsies in 1983. H.Pylori has been associated with various upper gastro-intestinal tract disorders (1) including gastritis, peptic ulcer and gastric malignancies. There is a paucity of literature regarding the study of morphological changes in H.Pylori associated gastritis, as H.Pylori colonized gastric mucosa is a distinct pathologic entity with a pathologic spectrum ranging from active chronic gastritis to erosions & frank ulcer. Thus the present study was undertaken to document the prevalence, morphological changes associated with H.Pylori colonization in gastric mucosa.

Material and Methods

A total of 100 patients who were clinically diagnosed as having acute or chronic gastritis were included for this study during the period December 1999 to December 2001 in BLDEA’s Shri B M Patil Medical college, Bijapur, India. Patient with H/O. tobacco chewing, alcohol intake and non-steroidal anti-inflammatory drug intake were excluded from this study.

Five endoscopic biopsies (2),(3) were taken from per patient for the study:• One for rapid urease from antrum along the greater curvature. • Two for histopathology from antrum and posterior wall of antrum • Other two for histopathology from anterior and posterior wall of mid-body (Table/Fig 1).

For Rapid Urease Test-
Biopsy specimen taken from the antrum were placed immediately in eppendorf tube containing 0.5 ml urease test reagent (4) and sealed with cap. Then the tubes were kept in incubator at 370c, development of red color (from yellow) were considered as positive test indicating the presence of H.Pylori (Table/Fig 1). Then the other 4 biopsies were processed for paraffin embedding, sections on the first slide were stained with routine Harri’s Haematoxyllin & Eosin stain (5). The histopathological evalution of stained section(first slide) were studied for H.Pylori associated gastritis (6),(7) (Table/Fig 2),(Table/Fig 3),(Table/Fig 4),(Table/Fig 5),(Table/Fig 6). The second slide was stained using the Giemsa technique for the demonstration of H.Pylori (3) (Table/Fig 7),(Table/Fig 8),(Table/Fig 9).

Results

In the present study a total of 100 patient presenting with symptoms of heart burn, epigastric discomfort, pain abdomen & vomitingwere subjected for endoscopy. On endoscopic examination signs of gastritis were observed. These cases were selected for further study. For each case rapid urease test was done in the operation theater itself. Taking histopathology as a final diagnosis morphological changes due to H.Pylori associated gastritis were studied in 100 cases & following results were obtained.

In total of 100 cases of chronic gastritis, common age of incidence was between 21-30 years with a male predominance. 48 were H.Pylori positive and 52 were H.Pylori negative by histopathology. Out of 48 H.Pylori positive cases, 43 were positive by both RUT & histopathology. But 3 were RUT positive & negative for H.Pylori by histopathology and 5 were negative by RUT but histopathology showed presence of H.Pylori (Table/Fig 10).

Thus specificity and sensitivity of Rapid urease test is Specificity – 94.23 %, Sensitivity – 89.58%. Morphological changes due to H.Pylori associated gastritis was noted as mentioned by Hui Pak212et. al (8) Among all irregular epithelial surface, loss of apical mucin are the two common features noted in all 48 cases of H.Pylori positive cases. In the normal gastric mucosa the epithelial surface is regular. Apical border in all the glands is smooth. In case of H.Pylori associated gastritis, the epithelial surface became irregular and uneven. Along with the loss of apical mucin characterized by nipping of apical bud with 100% cases.

77% were showing epithelial cell drop out which was characterized by pyknotic nuclei 58% were showing pit formation due to loss of apical mucin & cell drop out. These pits were deep at some places occupying crypts as well. 35% were showing micro erosionscharacterized by congested sub-epithelial layer, extravasated rbc’s, dense mixed inflammatory infiltrate composed of polymorphs and lymphocytes (Table/Fig 11). When RUT and histopathology were compared, out of 100 cases, 46cases were RUT positive & 54cases were RUT negative. Along with 48 cases were histology positive & 52 cases were histology negative. Statistically. no significant difference between urease test and histopathological demonstration of H.Pylori (P>0.05) (Table/Fig 12) (Table/Fig 4),(Table/Fig 5), (Table/Fig 6).

Discussion

In 1983, Warren & Marshall described the strong association between H.Pylori and chronic gastritis [9,10]. Previous studies hadfocused mainly on the inflammatory reaction of gastric mucosa colonized by H.Pylori . There were paucity of literature regarding the epithelial changes in gastritis till 1992.

Hui Pak et. al expanded this concept by studying the pathological changes of gastric mucosa colonized by H.Pylori on H & E stained tissue sections. They found out that the identifying H.Pylori was associated with epithelial degenerative changes greatly influence the identification of H.Pylori in gastric biopsies. The present study was designed to know the prevalence of H.Pylori associated gastritis in patients undergoing upper gastro-intestinal endoscopy in center who were clinically diagnosed as gastritis. Also to study the morphological changes in gastric mucosa associated with H.Pylori gastritis (11),(12).

A total of 100 cases of clinically diagnosed gastritis were included for this study. It is noted that Male patients with chronic gastritis were out numbered from female patients. The M:F ratio is 3:1. These results concure with the findings of Yoosuf H et al (13). Another author Taylor et.al reported prevalence rate of 43% in his study. In the present study prevalence of H.Pylori was 48% in patients attended upper gastro-intestinal endoscopiy units with gastritis. In a study of Singal A I quoated prevalence with a wide range of 31-84% (14).

The Sensitivity of RUT for diagnosis of H.Pylori infection is 89.58% and specificity of 94.23% respectively. These results correlates with Yoosuf et al. who got sensitivity of 89.83% & specificity of 100%. The only variation is towards specificity results in present study which could be due to patchy distribution of organism. In all 48 positive H.Pylori associated gastritis majority of epithelial degenerative changes included irregular surface epithelium & loss of apical mucin (100%). The least number of cases obtained were showing microerosion (35%) and second commonest finding of morphologic changes included cell drop out (77%) followed by formation of pits (58 %). These findings correlated with the study of Pak Hui et al (8). The only point is of micro erosions where we got less number of cases obtained in the present study compared with that of Pak Hui et al. study. This possibly could be due to picking ofcases more in early stages.

In the present study P>0.05 which was not significant indicating that there was no difference in diagnosing H.Pylorii associated gastritis by RUT & histopathology. It signifies that these two diagnostic methods play as a equal role in detection of H.PyloriFor these observation needs data for further study.

Conclusion

It is obvious that epithelial lesions described in our study are specific for H.Pylori colonization and are highly characterstic, easy to recognize. They were absent in H.Pylori negative gastric biopsies. Thus identifying H.Pylori associated epithelial changes can greatly facilitates the identification of H.Pylori in gastric biopsies. It is also obvious that the two diagnostic methods RUT & histopathology play an equal role in detection of H.Pylori. This needs further study to know importance of these diagnostic methods for better detection of H.Pylori associated lesion.

References

1.
Blaser MJ, Brown WR “Campylobacters and Gastroduodenal Inflammation”. Adv. Intern Med 1989;34 : 21-42.
2.
Goldman Harvey and Antonioli D.A. “ Mucosal biopsy of the esophagus, stomach and Proximal duodenum”. Hum Pathol 1982;13: 423-48.
3.
Gumee Donal Jr and Randali G Lee, “Laboratory methods for processing and interpretation of endoscpic gastro-intestinal biopsies”. Laboratory Medicine 1990:13-16.
4.
Lee A and Megraud F. “Helicobacter pylori: techniques for clinical diagnosis and basic research”. 1st edn ; W.B Saunders ; 1996:P-1-81.
5.
Bancroft JD, Stevens A, Ed. Theory and practice of histological techniques 4th ed, New York; Churchill Livingstone, 1996; P104, 306-07.
6.
Wyatt JI and Gray SF. “Detection of campylobacter pylori by histology”. In: Rathbone BJ, Heatley RV edn, Blackwell Scientific; Oxford, 1989; 63-68.
7.
Dixon MF, “Campylobacter pylori and chronic gastritis” In: Rathbone BJ, Heatley RV, End. Blackwell Scientific; Oxford, 1989 : 106-16.
8.
Hui Pak K, Chan WY, Cheung PSY, et al. “Pathologic changes of Gastric mucosa colonized by Helicobacter pylon”. Hum Pathol. 1992; 23:548-56.
9.
Whitehead R, Truelove SC and Gear MWL. “The histological diagnosis of chronic gastritis in fibreoptic gastroscope biopsy specimens”. J.Clin.Pathol 1972 ;25:1-11.
10.
Marshall BJ and Warren JR. “ Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration”. Lancet 1984; June 16: 1311-14.
11.
Marshall BJ. and Goodwin CS. “Revised Nomenclature of Campylobacter pyloridis”. Int. J of systematic Bacteriology 1987;37:P 68.
12.
Taylor DE, Hargreaves JA, Lai-king NG, et.al. “Isolation and characterization of campylobacter pyloridis from gastric biopsies”. Am J Clin Pathol 1987, 87:49-54.
13.
Mohamed Yoosuf H Usha AR. “A comparative study between Rapid urease (modified) clotest, Culture and Histopathologic examination for H.pylori with Acid peptic Disease”. Indian J Pathol. Microbiol 1995; 38 (4):349-354.
14.
Singal AK. “Helicobacter pylori Infection: Current Status”. Gastroenterology Today 1999;3:75-81.

DOI and Others

DOI: JCDR/2012/3803:1957

Financial OR OTHER COMPETING INTERESTS:
None.


Date Of Submission: Jan 03, 2012
Date Of Peer Review: Feb 03, 2012
Date Of Acceptance: Feb 21, 2012
Date Of Publishing: Apr 15, 2012

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