Platelets as an Extra-Hepatic Reservoir of Hepatitis C Virus RNA: A Prospective StudyCorrespondence Address :
Dr. Ekta Gupta,
D-1,Vasant Kunj, New Delhi-110070, India.
Introduction: In the era of Direct Acting Antiviral (DAA), elimination of HCV is possible but persistence in extra hepatic sites is hindrance. Detection of HCV has been reported in platelets, however its impact on DAA therapy not been studied.
Aim: Detection and quantification of HCV RNA from the platelets and compare with parallel plasma levels.
Materials and Methods: Whole blood collected from 62 chronic HCV infected patients. HCV RNA quantitation was done from paired plasma and platelet pool at baseline using commercial real time PCR assay (AbbottTM).
Correlation calculated by Bland-Altman analysis. Bivariate association and p-value calculated using chi-square tests.
Results: A total of 45 patients were viremic while 17 were non-viremic. Median plasma viral load was log105.7 (IQR: log103.55-6.7) IU/mL, median platelet viral load was log104.13 (IQR: log100-5.5) IU/mL. Genotype (GT) 3 (66.6%) was commonest followed by GT 1 (33.3%). Sustained Virologic Response (SVR) was attained in 39/45 patients (92.8%) patients while 3/45 patients (6.67%) were non-responders. Viral load at baseline in platelet pool was higher in non-responders when compared to those achieved SVR (median log104.46 vs. log103.27, p=0.456).
Conclusion: HCV-RNA was efficiently detected in platelets. Persistence in platelets could be one of the factors associated with treatment failure.
Direct acting antivirals, Real time polymerase chain reaction, Sustained virologic response
Pradheer Gupta, Manish Chandra Choudhary, Meenu Bajpai, Ekta Gupta. PLATELETS AS AN EXTRA-HEPATIC RESERVOIR OF HEPATITIS C VIRUS RNA: A PROSPECTIVE STUDY. Journal of Clinical and Diagnostic Research [serial online] 2018 December [cited: 2018 Dec 14 ]; 12:DM01-DM03. Available from
Date of Submission: Mar 21, 2018
Date of Peer Review: Apr 25, 2018
Date of Acceptance: Sep 08, 2018
Date of Publishing: Dec 01, 2018
Financial OR OTHER COMPETING INTERESTS: None.
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