Tobacco Usage and Serum Cotinine: A Hospital Based StudyCorrespondence Address :
Dr. Vaishali Satyajeet Pawar,
Assistant Professor, Department of Biochemistry, Krishna Institute of Medical Sciences, Deemed to be University,
Malakapur-415539, Karad, Maharashtra, India.
Introduction: Tobacco usage is mainly in the form of smoking and smokeless tobacco. Some studies commented that smokeless tobacco has high nicotine and toxic effect, but the studies with depictive data have been limited. Cotinine is one of the nicotine metabolite, which is used as a marker to quantify tobacco exposure.
Aim: To estimate serum cotinine (CTN) levels in tobacco smokers and smokeless tobacco users and to compare them with tobacco non-users.
Materials and Methods: This cross-sectional study was performed in 240 study subjects divided into four groups- only tobacco smokers (G1), only smokeless tobacco users (G2), dual tobacco smokers and smokeless tobacco users (G3), and tobacco non-users (G4). G2 was divided into G2a, G2b, and G2c. Serum CTN levels were estimated using enzyme linked immunosorbant assay (ELISA) kit. Chi-square test, unpaired t-test, ANOVA and regression analysis were used for statistical analysis.
Results: The mean serum CTN levels in groups G1, G2a, G2b, G2c, G3 and G4 were found to be 117.45 ng/mL, 138.09 ng/mL, 72.35 ng/mL, 100.34 ng/mL, 145.21 ng/mL and 5.28 ng/mL respectively. When the mean values were compared between the groups the values were found to be statistically highly significant. The finding suggests significantly higher CTN levels in tobacco users compared with tobacco non-users, also in tobacco chewers compared with tobacco smokers, as well as for dual tobacco smokers and smokeless tobacco users compared with smokers.
Conclusion: Dual tobacco smokers and smokeless tobacco users tend to have relatively high level of serum CTN, which suggests tobacco dependence.
Serum cotinine (CTN) levels, Smokeless tobacco users, Tobacco chewers, Tobacco smokers
Vaishali Satyajeet Pawar, Ajit V Sontakke, Krishnaji L Garud, Satyajeet Krishnarao Pawar. TOBACCO USAGE AND SERUM COTININE: A HOSPITAL BASED STUDY. Journal of Clinical and Diagnostic Research [serial online] 2019 February [cited: 2019 Feb 19 ]; 13:BC05-BC09. Available from
Date of Submission: Nov 04, 2018
Date of Peer Review: Nov 22, 2018
Date of Acceptance: Dec 29, 2018
Date of Publishing: Feb 01, 2019
FINANCIAL OR OTHER COMPETING INTERESTS: As declared above.
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2017: 134.54
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)