Serum Nitric Oxide and Plasma HbA1c Levels in Type 2 Diabetes Mellitus PatientsCorrespondence Address :
Shah Yasir Yousuf,
Jawaharlal Nehru Medical College, Belagavi, Karnataka, India.
Introduction: Diabetes Mellitus (DM) is a metabolic disorder characterised by hyperglycaemia and insufficiency of secretion or action of endogenous insulin. Glycated haemoglobin (HbA1c) is commonly used as reliable index to determine cumulative glycaemic history of preceding 2-3 months. Nitric Oxide (NO) is a potent vasodilator and an endothelium-relaxing factor. Low serum Nitric Oxide (NO) levels are known to be associated with endothelial dysfunction in Type 2 Diabetes Mellitus (T2DM).
Aim: To estimate the serum nitric oxide levels in type 2 diabetic patients and correlate it with serum HbA1c levels.
Materials and Methods: The cross-sectional study comprised of 100 (50 male and 50 female) T2DM patients who attended KLE’S Dr. Prabhakar Kore Hospital and Medical Research Centre, Belagavi, Karnataka, India. Fasting Plasma Glucose (FPG) was measured by Hexokinase method using Roche/Hitachi COBAS 6000 Fully Automated Analyser. HbA1c was estimated using a Bio-Rad D-10 HbA1c program and serum NO was estimated by Classical Griess Reaction. Data was analysed using independent t-test within the groups and Karl Pearson Correlation Coefficient in between the groups. The p<0.05 was considered as statistically significant.
Results: Statistically significant positive correlation was observed between NO and with HbA1c (r=0.7674) and NO with FBS (r=0.5688).
Conclusion: Hyperglycaemia increases NO levels in T2DM patients. Measurement of nitric oxide in early course of diabetes may help in preventing the progression and development of complications like endothelial dysfunction.
Diabetic complications, Endothelial dysfunction, Glycated haemoglobin, Griess reaction
Shah Yasir Yousuf, Sudha Ambiger, Dnyanesh Morkar. SERUM NITRIC OXIDE AND PLASMA HBA1C LEVELS IN TYPE 2 DIABETES MELLITUS PATIENTS. Journal of Clinical and Diagnostic Research [serial online] 2019 September [cited: 2019 Sep 16 ]; 13:BC04-BC06. Available from
Date of Submission: May 07, 2019
Date of Peer Review: May 15, 2019
Date of Acceptance: Jul 16, 2019
Date of Publishing: Sep 01, 2019
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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