Significance of Morphometric Evaluation of Pre-Malignant and Malignant Lesions of Uterine Cervix: A Cross-sectional Analytical StudyCorrespondence Address :
Dr. Kalyani Raju,
Professor and Head, Department of Pathology, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar-563101, Karnataka, India.
Introduction: Morphometric analysis of various parameters of the cells is done to classify the lesion as benign or malignant. It is an objective assessment which reduces the variability due to subjective visual assessment. Authors have done the nuclear morphometric analysis in ectocervix and endocervix tissue sections.
Aim: To evaluate the significance and utility of nuclear morphometry in pre-malignant and malignant lesions of uterine cervix.
Materials and Methods: This was a retrospective cross-sectional study done on 120 cases of cervix which were categorised histomorphologically into normal ectocervix, chronic cervicitis, Low-Grade Squamous Intraepithelial Lesion (LSIL), High-Grade Squamous Intraepithelial Lesion (HSIL), Squamous Cell Carcinoma (SCC), normal endocervix, chronic endocervicitis, Low-Grade Cervical Glandular Intraepithelial Neoplasia (LCGIN), Adenocarcinoma In Situ (AIS) and adenocarcinoma. The quantitative nuclear morphometry analysis was done and entered in Microsoft Excel sheet. Statistical analysis was done by using SPSS software 22.0 version. The p-value <0.05 was considered as statistically significant.
Results: In ectocervical cases, all the nuclear parameters as Mean Nuclear area (MNA), Mean Nuclear Perimeter (MNP), Minimum Nuclear Diameter (mND), Maximum Nuclear Diameter (MND), Mean Nuclear Compactness (MNC) and Mean Nuclear size (MNS) were statistically significant (p-value <0.05) except Mean Shape Factor (MSHF) where the p-value was 0.415. In the endocervical cases, only mND and MSHF were statistically significant.
Conclusion: Nuclear morphometry can be used as a tool in distinguishing the premalignant and malignant lesions in both ectocervical and endocervical tissue samples.
Cervical cancer, Cervical intraepithelial neoplasm, Morphometric study
Hajra K Mehdi, Kalyani Raju, SR Sheela. SIGNIFICANCE OF MORPHOMETRIC EVALUATION OF PRE-MALIGNANT AND MALIGNANT LESIONS OF UTERINE CERVIX: A CROSS-SECTIONAL ANALYTICAL STUDY. Journal of Clinical and Diagnostic Research [serial online] 2019 April [cited: 2019 Apr 23 ]; 13:EC05-EC07. Available from
Date of Submission: Nov 09, 2018
Date of Peer Review: Dec 25, 2018
Date of Acceptance: Feb 28, 2019
Date of Publishing: Apr 01, 2019
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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