Effects of Oral N-Acetylcysteine on the Haemato-Biochemical and Histopathological Changes on the Liver of Albino RatsCorrespondence Address :
Dr. Ervilla Dass,
Associate Professor, Department of Pharmacology, Smt. B.K Shah Medical Institute
and Research Centre, Sumandeep Vidyapeeth an Institution Deemed-to be University,
At. & Po. Piparia, Ta. Waghodia, Vadodara-391760, Gujarat, India.
Introduction: N-Acetylcysteine (NAC) is a direct precursor in the synthesis of intracellular Glutathione (GSH). NAC protects the liver by restoring the GSH levels or by acting as an alternate substrate for conjugation and hence, detoxification of the reactive metabolite of hepatotoxic drug.
Aim: To evaluate the effects of oral NAC on the haematobiochemical and histopathological changes on the liver of albino rats.
Materials and Methods: The study was carried out in animal house located at Sumandeep Vidyapeeth, Piparia. The healthy albino rats of either sex weighing 150-400 gm body weight were grouped into two for the experimental study and were housed in animal house for duration of 24 hours. Before initiation of any procedures; after overnight fasting; the albino rats belonging to Group I Control Group (n=6) were administered distilled water 10 mL/kg orally; Group II NAC treated (n=6) were administered NAC 450 mg/kg as a single oral dose. Blood samples were collected after 24 hours of treatment, to evaluate the effect of NAC on the haematobiochemical and histopathological changes on the liver. All results were expressed as Mean±SEM.
Results: NAC as a single oral dose of 450 mg/kg does not caused statistically significant changes in the serum enzymes levels. Moreover, histopathology showed normal appearance of the liver was similar to that of the control treated rats, with no change in the texture and liver tissue showed normal morphology.
Conclusion: NAC as a single oral dose of 450 mg/kg body weight has no toxic effect on the liver in albino rats.
Glutathione, Hepatoprotective agents, Liver damage, Liver function test
Date of Submission: Oct 22, 2020
Date of Peer Review: Nov 19, 2020
Date of Acceptance: Dec 09, 2020
Date of Publishing: Jan 01, 2021
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? NA
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Oct 26, 2020
• Manual Googling: Nov 27, 2020
• iThenticate Software: Dec 21, 2020 (19%)
ETYMOLOGY: Author Origin
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2017: 134.54
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)