Evaluation of IgG Antibody Response to Severe Acute Respiratory Syndrome Coronavirus-2 in Healthcare Workers in a Tertiary Care Centre, Chennai, IndiaCorrespondence Address :
Dr. Therese Mary Dhason,
Associate Professor, Department of Microbiology, Government Kilpauk Medical College, Kilpauk, Chennai-60010, Tamil Nadu, India.
Introduction: Globally, the Coronavirus Disease-2019 (COVID19) pandemic poses a high risk for Healthcare Workers (HCWs) who are among the population that is most vulnerable of being infected with Severe Acute Respiratory SyndromeCoronavirus-2 (SARS-CoV-2). With a prevailing pandemic such as COVID-19, it becomes important to understand the presence and persistence of antibodies in the serum of HCW, testing positive for COVID-19 on Reverse transcriptase Polymerase Chain Reaction (RT-PCR). An understanding of the prevalence of IgG antibodies against COVID-19 and the duration for which they are present in the serum will help in predicting the immune response of individuals against the disease.
Aim: To study the prevalence of COVID-19 IgG antibodies in laboratory confirmed COVID-19 RT-PCR positive symptomatic, asymptomatic and RT-PCR negative subjects.
Materials and Methods: The present longitudinal study was conducted from April to December 2020 with a sample size of 90 participants based on a pilot study. Blood sample was collected and serum was separated. Enzyme Linked Immunosorbent Assay (ELISA) was done to detect the presence of COVID-19 IgG antibody in serum. Chi-square test and Pearson correlation were used to find out the statistical significance of COVID19 IgG antibodies in COVID-19 positive and negative HCWs and the relationship between Cycle threshold values (Ct) and antibody levels, respectively.
Results: Fever with sore throat was the most common (33%) symptom. Chi-square test done to compare IgG among RTPCR positive and negative subjects showed p-value of <0.0001 which was significant. However, statistical significance was not found (p-value 0.9973) with respect to COVID-19 IgG antibodies in RT-PCR positive COVID-19 asymptomatic and symptomatic subjects. Mean antibody index in symptomatic and asymptomatic was 3.7743±1.9834 and 3.571±1.7961, respectively. Average number of days, the antibodies persisted was 25 days-266 days.
Conclusion: The prevalence of COVID-19 antibodies among RTPCR positive symptomatic, asymptomatic and RT-PCR negative subjects was 87.8%, 66.7% and 26.7%, respectively. The maximum number of days antibodies persisted was 266 days. Further studie will elucidate whether these antibodies prevent re-infection.
Breathlessness, COVID-19, Immune response, Immunoglobulins, Polymerase chain reaction, Transmission
Date of Submission: Feb 07, 2021
Date of Peer Review: Mar 24, 2021
Date of Acceptance: Apr 20, 2021
Date of Publishing: Jun 01, 2021
Author declarat ion:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Feb 09, 2021
• Manual Googling: Apr 19, 2021
• iThenticate Software: Apr 29, 2021 (11%)
Etymology: Author Origin
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2017: 134.54
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)