Evaluation of Protein Carbonyl and Vitamin C in Seminal Plasma of Infertile Male: A Hospital-based Study in Bengali PopulationCorrespondence Address :
Dr. Sandeep Jain,
Staff Doctors Hostel, Inside National Institute of Tuberculosis and Respiratory
Diseases (LRS TB Hospital, Mehrauli), Shree Aurobindo Sarani, New Delhi-110030, India.
Introduction: Male infertility has been coupled with the imbalance between production of Reactive Oxygen Species (ROS) and antioxidant (e.g.,: vitamin C) level. Elevated concentrations of ROS in the semen can lead to oxidative protein damage as they counter with the amino acids' side chains in the protein, leading to the production of carbonyl groups.
Aim: To assess if there is any difference of seminal plasma Protein Carbonyl (PC) and vitamin C level in male infertile and fertile subjects in the midst of their correlation with other relevant seminal parameters.
Materials and Methods: This was a hospital-based case-control study of a Bengali population. Semen samples of 124 males (Group A; 68 infertile males, Group B; 56 fertile males) were tested. Seminal fluid analysis was done with Makler counting chamber. PC and vitamin C were measured by Levin’s and Roe’s photometric methods respectively. To evaluate the differences in the mean ranks of these parameters Mann-Whitney U test was used.
Results: Both in group A and group B sperm count was positively correlated with motility and vitamin C but negatively correlated with PC at significant level p<0.05. Statistically significant differences of mean ranks of these parameters (sperm count: 52.82 and 74.26, motility: 52.10 Vs 75.13, PC: 76.57 and 45.41, vitamin C:55.99 and 70.40, Mann Whitney U:1245, 1197, 947 and 1461, respectively) between the two groups were found. Hence, indicate that in infertile subjects the balance between PC and vitamin C is disturbed.
Conclusion: Assessment of oxidative status may serve the clinician in additional management of idiopathic male infertility.
Antioxidant, Ascorbic acid, Reactive oxygen species, Sperm count
Date of Submission: Oct 06, 2021
Date of Peer Review: Oct 26, 2021
Date of Acceptance: Dec 14, 2021
Date of Publishing: Jan 01, 2022
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. No
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ETYMOLOGY: Author Origin
- Emerging Sources Citation Index (Web of Science, thomsonreuters)
- Index Copernicus ICV 2017: 134.54
- Academic Search Complete Database
- Directory of Open Access Journals (DOAJ)
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Popline (reproductive health literature)