Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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On Aug 2018

Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
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Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
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Aug 2018

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Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
On May 11,2011

Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
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Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."

Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
On April 2011

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.

Dr. Anuradha
On Jan 2020

Important Notice

Original article / research
Year : 2022 | Month : October | Volume : 16 | Issue : 10 | Page : SC06 - SC10 Full Version

Viral Aetiology of Severe Acute Lower Respiratory Tract Infection in Children from the Paediatric Intensive Care Unit at a Tertiary Care Hospital, Eastern India- A Retrospective Study

Published: October 1, 2022 | DOI:
Neha Ahuja, Somenath Gorain, Partha Pratim Pal, Mousumi Das

1. Senior Resident, Department of Paediatrics, Bhagirathi Neotia Woman and Child Care Centre, New Town, Kolkata, West Bengal, India. 2. Paediatric Intensivist, Department of Paediatrics, Bhagirathi Neotia Woman and Child Care Centre, New Town, Kolkàta, West Bengal, India. 3. Assistant Professor, Department of Community Medicine, Institute of Postgraduate Medical Education and Research, Kolkata, West Bengal, India. 4. Resident Medical Officer Cum Clinical Tutor, Department of Paediatrics, Chittaranjan Seva Sadan and Sishu Sadan Hospital of Obstetrics, Gynaecology and Child Health, Kolkata, West Bengal, India.

Correspondence Address :
Dr. Mousumi Das,
Skyline Lakeview Complex, H block, Flat 1B, Purbapara, Laskarpur, Kolkata, West Bengal, India.


Introduction: Viral infections are the common source of lower respiratory tract infection worldwide. Rapid and accurate detection of viral infections is important that can prevent antibiotic abuse. However, there are few studies determining viral aetiologies in paediatric subjects in Eastern India.

Aim: To study viral aetiology in children hospitalised with severe Acute Lower Respiratory Tract Infection (ALRTI) over a period of two years and to detect the impact of co-infection on severity and duration of hospitalisation.

Materials and Methods: This was a retrospective study based on electronic database in a tertiary care Paediatric Intensive Care Unit (PICU) of Bhagirathi Neotia Woman and Child Care Centre, Kolkata, West Bengal, India. Data was collected for all the patients who fulfilled the selection criteria and were admitted to PICU with ALRTI from March 2018 to March 2020. The medical records were reviewed in 51 patients with positive viral Reverse Transcription- Polymerase Chain Reaction (RT-PCR). Data was collected for the type of viruses infecting the subjects and whether there were single infection or co-infection. Co-infection is simultaneous infection with more than one virus. Statistical analysis was done using the IBM Statistical Package for Social Sciences (SPSS) Statistics for Windows, version 24.0 (Armonk, NY: IBM Corp). Student’s t-test and Chi-square test was used to compare single infection and coinfection. A p-value <0.05 was considered significant assuming the confidence interval of 95% as the level of significance.

Results: The highest positivity rate was 66.7% (34/51) observed in children in the age group 1-12 months. The common viruses detected were adenovirus seen in 26 subjects, Respiratory Syncytial Virus (RSV) in 16 subjects and Rhino/Enterovirus in 12 subjects. Clustering of cases were observed for RSV and influenza A in the colder months of the year. Single infection was seen in 74.5% of samples and co-infection in 25.5%. Comparison between single and co-infection with two viruses in terms of complications (p-value=0.163), average duration of PICU stay (p-value=0.70), C-reactive protein (p-value=0.952), procalcitonin (p-value=0.198), white blood cell count (p-value=0.737), absolute neutrophil count (p-value=0.612) were not significant.

Conclusion: This present study found that adenovirus and RSV were the leading viral pathogens for ALRTI in the PICU. Co-infection with multiple viruses compared with a single viral infection in a patient did not impact severity.


Adenovirus, Co-infection, Respiratory syncytial virus, Reverse transcription-polymerase chain reaction, Viral infection

Acute Lower Respiratory Tract Infections (ALRTIs) are among the three major causes of death in children less than 5 years worldwide with around 0.74 million under 5 deaths annually, accounting for 14% of all deaths of children under five years old but 22% of all deaths in children aged 1 to 5 years (1). In India, Pneumonia is the leading cause of death accounting for 19.7% of the proportion of deaths in the age group 1-4 years followed by injuries (19.3%) (2). Viruses accounted for 30-70% of ALRTI in many studies, the common ones being Respiratory Syncytial Virus (RSV), rhinovirus, influenza A virus and parainfluenza virus (3),(4),(5). Globally, the human respiratory syncytial virus is one of the main viruses causing lower Acute Respiratory Infections (ARI) and attributes to approximately 45% of the hospitalisations and deaths in children over 6 months old (3).

The commonly used diagnostic methods for virus detection in the past were viral culture, rapid antigen test and immunofluorescence assays but molecular assays, specifically multiplex Polymerase Chain Reaction (PCR) has gained attention now-a-days due to high sensitivity, specificity and ability to detect a broad range of viruses (6). Reverse Transcription-Polymerase Chain Reaction (RT-PCR) has also gained attention in the recent era because of its ability to detect newly emerging viruses like Coronavirus Disease-2019 (COVID-19) (7).

Simultaneous infection with multiple viruses at one point of time i.e co-infection is also not uncommon. There are some suggestions that the presence of more than one type of virus in the respiratory specimen may also affect the clinical presentation of respiratory tract infection and in turn, impact the severity and the hospital stay (8),(9). However, the relationship between co-infection and the severity of illness remains unclear.

There are many studies based on identifying the viral aetiology of ALRTI in the community (3),(5) and in overall hospitalised patients (4),(10),(11),(12) but, very few of them were studied solely in PICU patients (13). Also, there is hardly any epidemiological study on the ALRTI-related viral aetiologies in Eastern India due to diagnostic limitations and the cost of viral RT-PCR. The aim of this study is to detect the viral aetiology of severe ALRTI in children of Eastern India, aged 1 month to 5 years leading to PICU admission and to detect the impact of co-infection on severity and duration of PICU stay when compared with single infection.

Material and Methods

The present study was conducted retrospectively based on the electronic database for a duration of two years from March 2018 to March 2020 in a tertiary care hospital in Eastern India, Bhagirathi Neotia Women and Child Care Centre, New Town, Kolkata, West Bengal, India. The electronic medical records of the subjects fulfilling selection criteria with documented aetiological, demographic, clinical, laboratory and imaging data was entered in an MS Excel sheet followed by review and analysis for a period of 6 months from July 2021 to December 2021. A total of 51 eligible children’s records found with positive viral RT-PCR belong to the age group of 1 month to 60 months i.e. 5 years, admitted with severe Acute Lower Respiratory Tract Infection (ALRTI) in the Paediatric Intensive Care Unit (PICU) were retrospectively investigated. The term severe ALRTI was used for the ALRTI fulfilling our PICU admission criteria. The sample size was taken as total eligible study subjects within the study period based on the records available.

The Institutional Ethics Committee (IEC) approval from the IEC of Bhagirathi Neotia Women and Child Care Centre, New town, Kolkata, India, was taken on 30th March 2021. As this was a recordbased study, informed consent was not a possibility.

Inclusion criteria: All patients aged 1 month to 5 years who were admitted to the PICU and had positive viral respiratory panel were included in the study. Admission to the PICU was guided by the local PICU admission policy after assessing children according to the Paediatric Assessment Triangle (PAT) (14) and that includes

1. Critically ill and unstable patient
2. Patient requiring continuous monitoring
3. Respiratory failure or impending respiratory failure and severe respiratory distress
4. Requirement of high flow nasal cannula, invasive and non invasive ventilation for maintaining target saturation
5. Need for inotropic support
6. Children with multiorgan dysfunction

Exclusion criteria: Children with nosocomial infections, positive blood culture in admission samples, bacterial and viral coinfection, previous recurrent episodes of ALRTI, chronic illness (neurological disorder, heart conditions, chromosomal disorders and immunodeficiency) and other co-morbidities were excluded from the study. Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) (COVID-19) infection was also excluded.

Sample Collection and Processing

In this healthcare setting, each and every subject was clinically assessed by an on-duty paediatric intensivist and admitted according to the hospital PICU admission criteria. The nasopharyngeal swab was routinely collected by a qualified paediatrician (on-call) within 24 hours of admission and was send for all the patients presenting to PICU with severe respiratory symptoms. The real-time RT-PCR used in the institution can detect 10 viruses after processing the nasopharyngeal samples. In this present study, the length of PICU stay, inflammatory markers and associated complications as the surrogate marker was considered for assessing the severity between viral co-infection and single infection.

The parameter, 'co-Infection' was defined as the simultaneous detection of more than one virus in one clinical specimen. In this study, a comparison was made between the ‘co-infection with two viruses’ and ‘single viral infection’. The differences between ‘single infection’ and ‘co-infection with two viruses’ were studied in terms of duration of PICU stay, survival, inflammatory markers, presence of complications like Acute Respiratory Distress Syndrome (ARDS), Pleural effusion, septic shock, Multiorgan Dysfunction Syndrome (MODS), chronic lung disease, hepatitis, Haemophagocytic Lymphohistiocytosis (HLH), pneumothorax, empyema and encephalopathy.

Statistical Analysis

As per selection criteria, the data was collected, collated and entered in an Microsoft Excel sheet and analysed using the data (both variables and their values) were coded into an alpha-numeric format for concealment with few designated persons having the coding key. Categorical variables are presented as percentages and continuous variables are presented as mean and standard deviation or median with interquartile range. Student's t-test and Chi-square test was used to compare clinical and laboratory parameters between single infection and co-infection. The confidence interval of 95% was assumed as the level of significance so that the p-value <0.05 was considered statistically significant.


(Table/Fig 1) shows the patient’s demographic and clinical data of total 51 subjects, in which the mean age was 15±16.3 months and the median age was 10 (IQR 6,14.5) months.

(Table/Fig 2) demonstrates the number of different types of viruses detected in the nasopharyngeal RT-PCR samples.

Out of a total 51 study subjects, 38 (74.5%) children had single infection and 13 (25.5%) had co-infection with multiple viruses. Co-infection with two viruses was seen in 10 (19.6%) samples, three viruses in 2 (4%) samples and four viruses in 1 (2%) samples. (Table/Fig 3) shows the distribution of single viral infection and co-infection patterns amongst the study population. Adeno viral aetiology was the most common single infection whereas Adenovirus plus Respiratory Syncytial Virus (RSV) and Adenovirus with Influenzae A were common amongst co-infections with two pathogens. The present study just compared single infection with co-infection with two viruses as the co-infection with two viruses was the most common co-infection found in the samples. (Table/Fig 4) demonstrates the differences between single infection and co-infection with two viruses in terms of duration of stay, inflammatory markers, and presence of complications like Acute Respiratory Distress Syndrome (ARDS), pleural effusion, septic shock, Multiorgan Dysfunction Syndrome (MODS), chronic lung disease, hepatitis, Haemophagocytic Lymphohistiocytosis (HLH), pneumothorax, empyema and encephalopathy. Statistical analysis of present study data showed no statistical significance in terms of severity (which was measured as the duration of PICU stay, Infection markers like absolute neutrophil count, CRP, procalcitonin and complications seen) between single infection and co-infection.


This study was conducted to find out the viral aetiology of severe ALRTIs in children admitted to the PICU and to identify the effect of co-infection on severity. The median age of presentation was 6 months and the incidence of severe ALRTI decreased with age being more in infancy and then decreasing as the age increased. This may be because infancy is a time of increased disease susceptibility and severity. Of the 51 patients, 62.7% were males and 37.3% were females; the reason for this discrepancy is unclear but may be due to a reporting bias where parents seek care for boys more than girls. Almost 3/4 of the infants admitted were not having proper breastfeeding and that could be a predisposing factor for infection (15),(16). The common presentations of these ALRTIs were bronchiolitis and pneumonia. This classification is sometimes difficult because X-ray findings can vary due to interobserver bias in clinicians. In our study clustering of cases was seen in the winter months and that can be due to a decrease in innate immunity defence mechanisms such as mucociliary clearance, leading to increased susceptibility to viral infections (17),(18),(19).

Adenovirus as the leading viral pathogen causing severe ALRTI hospitalisation in Eastern India: While studying the retrospective data for 2 years authors clarified the burden of virus infection causing PICU admission. The most commonly detected virus in our study was adenovirus followed by RSV and influenza A. This was in contrast to the study conducted by Duyu M and Karakaya Z, on critically ill 115 children in the PICU unit of a tertiary care hospital in Istanbul where they found RSV as the most common virus (36.5%) followed by hRV and Human Bocavirus (HBoV) sharing equal frequency (27%) (13). Other hospitalbased studies also showed RSV as the most common pathogen detected (3),(4),(10),(11),(12) (Table/Fig 5). This can be explained by the local topographic and latitude location of Kolkata where the study took place. Kolkata because of its high average humidity level may give a favourable environment for adenoviruses as this virus is much more stable in areas with high humidity levels (18).

Respiratory virus co-infections and their interaction in the development of LRTI s: The co-infection rate in this study was 25.5% and there was no statistical difference between co-infection with two viruses and single infection in terms of inflammatory markers, duration of PICU stay and complications which were comparable to the study by Lin CY et al., which was conducted in MacKay Memorial Hospital, Taipei, Taiwan, where a co-infection rate of 25.8% was found and they further compared the clinical manifestations of patients in which either no viruses, a single infection or co-infections were detected (20). The age, body weight, duration of hospitalisation, Intensive Care Unit (ICU) stay, White Blood Cell (WBC) counts, and C-Reactive Protein (CRP) levels were not significantly different. A meta-analysis and systematic review for clinical disease severity of respiratory viral co-infection versus single viral infection which was concluded over 21 studies involving 4,280 patients showed no significant differences in Length of Hospital Stay (LOS) (mean difference-0.20 days, 95% CI- 0.94- 0.53, p-value=0.59), or mortality (RR 2.44, 95% CI 0.86, 6.91, p-value=0.09) in subjects with viral co-infections compared to those with a single viral infection (16).

The present study was not be able to compare mortality in single versus co-infection as some of the patients who took Leave Against Medical Advice (LAMA) were in critical condition, therefore, the outcome in that respect was inconclusive. A study by Yoshida LM et al., which was conducted in Vietnam found that co-infection of RSV with certain respiratory viruses increased the risk for LRTIs that is due to synergistic interactions of viruses (8). Another study by Semple MG et al., was conducted on children less than 2 years with bronchiolitis and found to have a 10-fold increase in Relative Risk (RR) of admission to a paediatric ICU for mechanical ventilation (9). Different study designs, detection methods and population diversity may be the reason behind this inconclusiveness.

Viral infections are ubiquitous and there is no clear-cut clinical difference between viral and bacterial infection leading to unwarranted use of antibiotics in all cases presented with ALRTI and antibiotic treatment does not help in patients having primarily viral ALRTI. Moreover, correct diagnosis of viral agents can lead to effective therapy in cases of certain viral infections like oseltamivir in influenza infection; also contributes to effective infection control measures and isolation care. Also, detection of the respiratory virus could enable estimation of local epidemiology, therefore, help in clinical judgements of practitioners.


Firstly, this study has limitations due to the small sample size. Secondly, the multiplex RT-PCR was used here to detect nine viruses, which may have chances that some of the viruses which were not detected may be missed. Lastly, respiratory viral infections are known to predispose to secondary bacterial pulmonary infections, and thus can result in substantial confounding when comparing a single viral infection with viral co-infection.


This study provides an overview of viral causes of severe ALRTI as awareness of pathogen leads to accurate diagnosis and management; however, a large multicentre study is required for determining the causative viral agents leading to PICU admission as a clear picture of their prevalence in PICU can help to reduce unnecessary antibiotic abuse. Adenovirus is one of the most common viral cause of ALRTI in PICU and need specific attention for the development of any vaccine or antiviral agent against it. Also, the presence of co-infection with two viruses did not have any impact on the severity when compared with single viral infection. Future studies should employ stratified analysis where the effects of specific pairs of viruses are studied so as to find the type of virus pairs which increase or decrease disease severity.


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DOI and Others

DOI: 10.7860/JCDR/2022/57925.17026

Date of Submission: May 28, 2022
Date of Peer Review: Jun 21, 2022
Date of Acceptance: Aug 16, 2022
Date of Publishing: Oct 01, 2022

• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. NA

• Plagiarism X-checker: Jun 03, 2022
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