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On Sep 2018




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"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."



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Saraswati Dental College
Lucknow
On Sep 2018




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On Aug 2018




Dr. Arundhathi. S
"Journal of Clinical and Diagnostic Research (JCDR) is a reputed peer reviewed journal and is constantly involved in publishing high quality research articles related to medicine. Its been a great pleasure to be associated with this esteemed journal as a reviewer and as an author for a couple of years. The editorial board consists of many dedicated and reputed experts as its members and they are doing an appreciable work in guiding budding researchers. JCDR is doing a commendable job in scientific research by promoting excellent quality research & review articles and case reports & series. The reviewers provide appropriate suggestions that improve the quality of articles. I strongly recommend my fraternity to encourage JCDR by contributing their valuable research work in this widely accepted, user friendly journal. I hope my collaboration with JCDR will continue for a long time".



Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
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Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
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Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.
Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
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In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
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Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."



Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2023 | Month : March | Volume : 17 | Issue : 3 | Page : ZC20 - ZC23 Full Version

Environment to Store Alginate at its Best: An In-vitro Study


Published: March 1, 2023 | DOI: https://doi.org/10.7860/JCDR/2023/59033.17595
Ankita Pal, Mukesh Kumar Singhal, Divya Jha, Megha Chopra, Kunal Yadav

1. Assistant Professor, Department of Prosthodontics and Crown and Bridge, School of Dental Sciences, Sharda University, Greater Noida, Uttar Pradesh, India. 2. Professor and Head, Department of Prosthodontics and Crown and Bridge, Institute of Dental Sciences, Bareilly, Uttar Pradesh, India. 3. Postgraduate Student, Department of Prosthodontics and Crown and Bridge, Institute of Dental Sciences, Bareilly, Uttar Pradesh, India. 4. Assistant Professor, Department of Prosthodontics and Crown and Bridge, Manav Rachna Institute of Dental Sciences, Faridabad, Haryana, India. 5. Postgraduate Student, Department of Pedodontics and Preventive Dentistry, Azamgarh Dental College, Azamgarh, Uttar Pradesh, India.

Correspondence Address :
Dr. Ankita Pal,
Assistant Professor, Department of Prosthodontics and Crown and Bridge, School of Dental Sciences, Sharda University, Greater Noida-201310, Uttar Pradesh, India.
E-mail: dr.ankitapal03@gmail.com

Abstract

Introduction: Irreversible hydrocolloid alginate impressions are among the widely used impression materials in dentistry but they suffer from one major drawback, i.e., dimensional change after setting. Because of clinical limitations, impressions are poured to make cast after a time delay which is so long as compared to the recommended period. This can be reduced by storing the impression material in different storage environments.

Aim: To assess the impact of the storage environment on the dimensional accuracy of alginate impression material.

Materials and Methods: In this in-vitro study, 70 samples were made with chromatic alginate impression material according to the manufacturer’s guidelines and divided into seven groups (Exposed to atmosphere, Airtight container in the refrigerator, 2% NaCl (Sodium Chloride) solution, Air-tight container, Distilled water, 1% K2SO4 (Potassium Sulphate) solution and Mixture of 2% NaCl and 1% K2SO4 Solution). Samples were made with stainless steel die fabricated according to ADA specification 18 and dimensions were noted after 24 hours of storage. Distance between the cross lines was calculated using profile projector. ANOVA Test and Tukey Test were applied to compare the mean dimensional change of seven groups and to compare the difference in mean dimensional change between the groups.

Results: Samples kept in a solution of 1% K2SO4 and 2% NaCl solution had no significant dimensional change (F-value 2026.0, p-value <0.001), whereas samples stored in open-air resulted in the greatest dimensional change (Group 7 > Group 6 > Group 3 > Group 4 > Group 2 > Group 5 > Group 1).

Conclusion: The impressions which are kept in a combined solution of 1% K2SO4 and 2% NaCl have shown the best impact on the dimensional accuracy of alginate impression materials. However, these can also be seen in 1% K2SO4 or in 2% NaCl solutions, respectively.

Keywords

Dimensional change, Distilled water, Irreversible hydrocolloid impression materials, Potassium sulphate solution, Sodium chloride solution

Alginate impression materials are the most extensively used dental materials in dentistry (1). These impression materials were developed in the late 1930s (2). Because of its admissible accuracy, accessibility, affordable cost, and easy handling, this material is used for many purposes like fabrication of a study model, provisional prosthesis, custom made trays, appliances, and a definitive cast for complete denture fabrication in cases with severe undercuts, removable partial denture and for the maxillofacial prosthesis (1),(3).

This impression material is supplied in the form of powder which contains potassium or sodium alginate, calcium sulfate, tri-sodium phosphate, diatomaceous earth, potassium titanium fluoride, zinc oxide, etc. When powder and water are mixed a sol is formed through a reaction between calcium sulfate and sodium or potassium salts of alginic acid. Then alginic salts cross-linked into a flexible matrix by replacement of monovalent sodium and potassium ions with calcium ions (4).

The alginate impression materials suffer from a major disadvantage, i.e. dimensional change after setting of the material which is affected by numerous factors which include the composition of a material, the environment in which impressions are stored, and duration of storage before pouring (1).

Irreversible hydrocolloid impressions materials tend to shrink if they are stored in the air; whereas, if they come in contact with water they swell as a result of imbibitions. Minor changes have also been observed when stored in 100% relative humidity (1).

To achieve maximum accuracy, these impressions should be poured instantly or within 12 minutes. Because of certain limitations, in many cases, impressions are poured at a delayed interval as compared to the normal recommended period. Nowadays, to overcome these, advancements have been done to increase the storage time of hydrocolloid impression materials (1). Recently, extended storage alginate impression materials have claimed that impressions can be stable if poured within 100 hours (5).

The time and medium in which the material is stored before pouring is a significant factors in the dimensional stability of these materials, thus in the resulting stone casts (6). Studies on different storage media are limited (4),(5).

Thus, this in-vitro study aimed to evaluate the dimensional accuracy of alginate impression material through different storage conditions.

Material and Methods

This in-vitro study was conducted in the Department of Prosthodontics, Crown and Bridge Institute of Dental Sciences Bareilly, Uttar Pradesh, India from October 2016 to April 2019 to evaluate the best environment for storage of alginate impression material in different mediums. The study was undertaken after obtaining Ethical Clearance from the Ethical Committee at SRMS University Bareilly (ME/SRMS/2018-2019/135).

Study Procedure

Chromatic alginate (Vignette, Densply), 2% NaCl solution, 1% K2SO4 solution, distilled water were used in the study. The instruments and type of equipments used in the study were stainless steel die according to ADA specification 18, Profile Projector (MEERA METZER), clamp, rubber bowl, spatula, metal plate, stainless steel die, air tight containers. ((Table/Fig 1): Armamentarium, (Table/Fig 2):
Profile Projector).

A stainless steel die was fabricated according to ADA Specification No. 18 which consisted of ruled block, a mold and a riser. The ruled block had two horizontal and three vertical lines. The vertical lines had a depth of 20 μm, 50 μm and 75 μm, respectively and the distance between each line was 2.5 mm. The distance between the two horizontal cross lines was 25 mm.The riser was 29.97 mm in diameter and 3 mm in height (5). ((Table/Fig 3): Die According to ADA Specification 18).

Alginate was mixed according to the manufacturer’s instructions and placed into the mold. Then the mold was covered with a thin sheet of polyethylene followed by a metal plate. Sufficient force was applied to extrude excess impression material and bring the sheet in contact with the mold. This procedure was performed at a room temperature of 29°C.

To ensure a complete setting, the manufacturer’s instructions were followed and the mold and test block were separated. After setting, the impression was pressed out of the mold using the riser. A numeric coding system was used to identify the sample. Seventy samples were made and they were divided into seven groups; group 1 to group 7. The descriptions of groups are as follow:

Group 1- Exposed to the atmosphere
Group 2- Air tight container in refrigerator
Group 3- Solution of NaCl at 2% concentration (4)
Group 4- Air-tight container
Group 5- Distilled water
Group 6- Solution of K2SO4 at 1% concentration (4)
Group 7- Solution of 2% NaCl and 1% K2SO4

Distance between the cross lines, on the ruled metal block, was measured to the nearest 0.005 mm and recorded as reading A ((Table/Fig 4): Alginate sample for dimensional accuracy, (Table/Fig 5): Alginate samples for dimensional accuracy before and after 24 hours). Then again this distance was measured after 24 hours of storage in different environments. These measurements were recorded for 10 samples as B-1 to B-10 in each group to same all the group, respectively. The dimension of lines was measured at the nearest 0.005 mm by using a Profile Projector (MEERA METZER) and the readings were recorded accordingly ((Table/Fig 6): Measurements of distance between crosslines).

All the obtained observations were collected and dimensional change was calculated as follows:

Dimensional change%=(A-B)/A×100 (5)

Statistical Analysis

The results were subjected to statistical analysis in SPSS software (Windows version 17.0) to evaluate the dimensional accuracy of alginate in different storage mediums. ANOVA test and Tukey test were applied to compare the dimensional accuracy of alginate in each group.

Results

The dimensional change of the seven groups is summarised in (Table/Fig 7). The mean dimensional change of Group 7 was the least followed by Group 6, Group 3, Group 4, Group 2, Group 5, and Group 1 the highest. Comparing the mean dimensional change of seven groups, ANOVA showed significantly different dimensional changes among the groups (F=2026.0, p<0.001) (Table/Fig 8).

Further, comparing the difference in mean dimensional change between the groups, the Tukey test showed significantly (p<0.001) different and lower dimensional change of Group 2 (90.3%), Group 3 (91.6%), Group 4 (90.4%), Group 5 (87.3%), Group 6 (92.3%) and Group 7 (94.3%) as compared to Group 1 (Table/Fig 9).

Further, it was also found significantly (p<0.001) different and lower in both Group 6 (39.0%) and Group 7 (55.0%) as compared to Group 5. Further, it also lowered significantly (p<0.05 or p<0.01) in Group 7 as compared to both Group 2 (41.0%) and Group 4 (40.3%). Moreover, it also lowered significantly (p<0.01) in Group 3 (33.8%) as compared to Group 5. However, it is not differed (p>0.05) between other groups i.e., found to be statistically the same.

Discussion

Based on the current study, the result states that the combination of 2% NaCl and 1% K2SO4 was the best medium for the storage of alginate impression material. The samples stored in NaCl solution, K2SO4 solution, or in air tight containers were also showing minimal dimensional changes whereas the samples stored in open atmosphere showed the highest dimensional changes.

The impact of storage of impression material in K2SO4 and NaCl solutions can be explained by Hofmeister or lyotropic series in which the ions of metallic salts were arranged in order of their decreasing ability to precipitate lyophilic substances from colloidal dispersion (anion order: SO-24 >C2H3O2- > Cl->NO3- >ClO3- >I- >CNS; cation order: Mg2+ >Ca2+ >Sr2+ >Ba2+>Li+ >Na+ >K+). As the coagulation power/extent of coagulation of gel is high, volume will decrease and thus shrinkage takes place. The surface stabilization for a limited period of time can be achieved by immersion in dilute aqueous solution of 2% NaCl and 1% of K2SO4 or a combination of both (4). Alginate contains approximately 70% water due to which this set alginate is subject to syneresis. As a result, the network of interlinked molecules constricts, and water is expelled from the interstitial spaces between the alginate chains. This results in shrinkage even though the material is also still hygroscopic and capable of absorbing water via imbibition, which results in the expansion of the impression (7). There is no universal standard for clinically acceptable changes of these impression materials. The inaccuracy up to a range of 78 μ μm and 50 μm, respectively are acceptable and also investigated the accuracy between the dies and the crown (8).

The effect of different fixing solutions on the irreversible hydrocolloid impression materials has been studied about composition and concentration by Skinner EW et al., (9). They concluded that the materials are dimensionally stable if they are stored in an environment which is of 100 percent relative humidity (9).

In distilled water the impression samples initially expands, because of the absorption of water to reach equilibrium. But after few hours because of the negative effect (decrease in weight) heavier components from the gel are substituted with lighter constituents (H+ ions) of water (4). In despite of its several advantages, dimensional changes of alginate is one of the major concerns (10). If an impervious film of some nature can be formed over the surface of the impression, no molecular exchange can take place between the atmosphere and the impression material, with no resulting imbibitions, syneresis and dimensional change (9). Findings of studies on different storage medias are compared in (Table/Fig 10) (4),(5),(11),(12),(13).

Limitation(s)

The limitation of this study is that the flat surface of alginate was used to record the prescribed, disregarding the possibility of dimensional changes in three dimensions. Moreover, the tested alginate samples do not represent clinically relevant forms as in real impressions in clinical practice.

Conclusion

Based on the results of study, it is concluded that keeping the impressions in the solution of 1% K2SO4 and 2% NaCl is the best method for maintaining the dimensional accuracy of alginate. It can also be stored in 1% K2SO4, in 2% NaCl, solutions for about 24 hours, facilitating the shifting to distant laboratories from the clinics. However, further research can be done to study the dimensional changes in clinical situation.

References

1.
Rohanian A, Shabestari GA, Zeighami S, Samadi MJ, Shamshiri AR. Effect of storage time of extended- our and conventional alginate impressions on the dimensional accuracy of casts. J Dent (Tehran). 2014;11(6):655-64.
2.
Culhaoglu AK, Zaimoglu A, Dogan E, Ozkir SE. The influence of different mixing methods on the dimensional stability and surface detail reproduction of two different brands of irreversible hydrocolloids. Eur J Gen Dent. 2014;3(1):17-21. [crossref]
3.
Shillingburg HT. Fundamentals of Fixed Prosthodontics. 4 th ed. Chicago: Quintessence Publishing Co; 2012. Impressions; pp. 291-306.
4.
Jayaprakash K, Nandish B, Shetty K, Voddya S, Prabhu S. Impact of storage environments on the dimensional stability of irreversible hydrocolloid alginate impression used in dentistry. Int J Health Rehabil Sci. 2014;3(1):24-29. [crossref]
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Walker MP, Burckhard J, Mitts DA, Williams KB. Dimensional change over time of extended-storage alginate impression materials. Ang Orthodontist. 2010;80(6):1110-15. [crossref] [PubMed]
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Kusugal P, Chourasiya RS, Ruttonji Z, Astagi P, Nayak AK, Patil A. Surface detail reproduction and dimensional stability of contemporary irreversible hydrocolloid alternatives after immediate and delayed pouring. Contemp Clin Dent. 2018;9(1):20-25. [crossref] [PubMed]
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Todd JA, Oesterle LJ, Newman SM, Shellhard WC. Dimensional changes of extended-pour alginate impression materials. Am J Orthod Dentofacial Orthop. 2013;143(4):55-62. [crossref] [PubMed]
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Farzin M, Panahandeh H. Effect of pouring time and storage temperature on dimensional stability of casts made from irreversible hydrocolloid. J Dent (Tehran). 2010;7(4):179-84.
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Skinner EW, Pomes CE. Dimensional stability of alginate impression materials. J Am Dent Assoc. 1946;33:1253-60. [crossref] [PubMed]
10.
Wadhwa SS, Mehta R, Duggal N, Vasudeva K. The effect of pouring time on the dimensional accuracy of casts made from different irreversible hydrocolloid impressions. Contemp Clin Dent. 2013;4(3):313-18. [crossref] [PubMed]
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DOI and Others

DOI: 10.7860/JCDR/2023/59033.17595

Date of Submission: Jul 12, 2022
Date of Peer Review: Aug 10, 2022
Date of Acceptance: Nov 22, 2022
Date of Publishing: Mar 01, 2023

AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. Yes

PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Jul 16, 2022
• Manual Googling: Aug 12, 2022
• iThenticate Software: Nov 21, 2022 (19%)

ETYMOLOGY: Author Origin

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