A Study On The Detection Of Micrometastases In The Cervical Lymph Nodes Of Oral Squamous Cell Carcinomas By Serial Sectioning
A Ravi Prakash, MDS
Department of Oral and maxillofacial pathology,
G Pulla Reddy Dental College and Hospital,
Kurnool - 518002,
Andhra Pradesh, India.
Mobile No. +919448457595
Background: Metastatic deposits in the regional lymph nodes have always been a subject of great interest in oncology. There is a need for the detection of micrometastases in the lymph nodes in oral carcinomas by serial sectioning, so that any missed tumour cells in the routine single sectioning technique could be detected by this. The present study was aimed at the detection of micrometastases in the cervical lymph nodes in oral squamous cell carcinomas- by serial sectioning.Materials and Methods: This study was done on 16 cases with 119 lymph nodes which measured 1cm or less in greatest diameter, by a single section routine method and this was compared with serial sectioning at 100Âµm intervals. All the 2269 sections were stained with the routine Hematoxylin and Eosin staining for the detection of micrometastases.Results: The detection of micrometastases by the serial sectioning method was 2.03%. The percentage was the same for both the one section and the serial sectioning methods.Conclusion: The serial sectioning of the lymph nodes did not reveal any other detection than that was revealed by the one section method. But definitely, the serial sectioning method appears to be the best feasible method to evaluate micrometastases.
Key words: Carcinoma, Lymph nodes, Micrometastases