Year :
2021
| Month :
June
| Volume :
15
| Issue :
6
| Page :
BC09 - BC12
Full Version
Association of Islet Amyloid Polypeptide and Inflammatory Markers in Type 2 Diabetes Mellitus
Published: June 1, 2021 | DOI: https://doi.org/10.7860/JCDR/2021/48524.14968
Zunjarrao Ganpat Badade , Yogita Mahendra Shinde , Shibban K Kaul , Sandeep Rai , Sameer Kadam
1. Professor, Department of Biochemistry, MGM Medical College, Navi Mumbai, Maharashtra, India.
2. PhD Scholar, Department of Biochemistry, MGM Medical College, Navi Mumbai, Maharashtra, India.
3. Professor, Department of CVTS, MGM Medical College, Navi Mumbai, Maharashtra, India.
4. Professor, Department of General Medicine, MGM Medical College, Navi Mumbai, Maharashtra, India.
5. Professor and Head, Department of General Surgery, MGM Medical College, Navi Mumbai, Maharashtra, India.
Correspondence Address :
Yogita Mahendra Shinde,
PhD Scholar, Department of Biochemistry, MGM Medical College, Navi Mumbai, Maharashtra, India.
E-mail: yogis8stars@rediffmail.com
Abstract
Introduction: Islet Amyloid Polypeptide (IAPP) is co-synthesised and co-secreted with insulin by islets of pancreatic β-cells, its increased concentration in individuals with Type 2 Diabetes Mellitus (T2DM), may involve in inflammatory processes.
Aim: To study and find the association of IAPP with inflammatory markers including high-sensitivity C-reactive protein (hsCRP), Tumour Necrosis Factor (TNF)-a and Interleukin (IL)-6 in patients with T2DM.
Materials and Methods: This was a cross-sectional study, conducted from, December 2015 to December 2019, on 262 subjects (30-60 years, 147 males and 115 females), including 131 healthy controls and 131 known cases of T2DM, Fasting blood glucose and glycated haemoglobin (HbA1c) was estimated by commercially available kit, and serum fasting insulin, IAPP, hsCRP, TNF-α, and IL-6 were analysed by ELISA. Insulin Resistance (IR) and insulin sensitivity were calculated. Statistical analysis was done with Statistical Package Social Sciences (SPSS) version 24. The descriptive statistics were expressed as Mean±SD, Student t-test was applied, and p≤0.05 was considered as statistically significant and highly significant at =0.01 at 95% CI. The relationship between IAPP and other variables were done by using Spearman Rank correlation (r).
Results: Circulatory level of IAPP was positively associated with serum hsCRP (r=0.312, p≤0.05) in T2DM. The elevated levels of IAPP (21.3±10.54 pmol/L), insulin (11.74±3.91 µU/mL), Homeostatic Model Assessment for Insulin Resistance (HOMAIR) (5.11±2.01) and inflammatory markers including hsCRP (3.44±1.77 mg/L), TNF-a (29.5±4.7 pg/mL) and IL-6 (20.2±5.2 pg/ mL) p≤0.05 with poor glycaemic control (HbA1c 8.78±2.30%, p<0.001), and reduced insulin sensitivity {Quantitative Insulin Sensitivity Check Index (QUICKI) 0.31±0.02, p≤0.05} were compared with healthy control; indicates that hyperamylinemia may induce inflammation in individuals with T2DM.
Conclusion: Elevated degree of IAPP can be an important predictor and indicator of inflammatory processes and IR in uncontrolled diabetes.
Keywords
Hyperamylinemia, Inflammation, Poor glycaemic control, high-sensitive C-reactive protein, Tumor Necrosis Factor, Interleukin-6
10.7860/JCDR/2021/48524.14968
Date of Submission: Jan 14, 2021
Date of Peer Review: Mar 11, 2021
Date of Acceptance: Apr 20, 2021
Date of Publishing: Jun 01, 2021
AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. NA
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Jan 15, 2021
• Manual Googling: Apr 19, 2021
• iThenticate Software: Apr 29, 2021 (6%)
ETYMOLOGY: Author Origin
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